DIG-11 insecticidal cry toxins
View Patent ↗DIG-11 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
1. An isolated polypeptide comprising residues 142 to 664 of SEQ ID NO:2, wherein said polypeptide has insecticidal activity.
2. The isolated polypeptide of claim 1 comprising residues 1 to 664 of SEQ ID NO:2, wherein said polypeptide has insecticidal activity.
3. The isolated polypeptide of claim 1 comprising residues 142 to 1164 of SEQ ID NO:2, wherein said polypeptide has insecticidal activity.
4. The isolated polypeptide of claim 1 comprising the amino acid sequence of SEQ ID NO:2.
5. A plant comprising the polypeptide of claim 1 .
6. A plant comprising the polypeptide of claim 2 .
7. A plant comprising the polypeptide of claim 3 .
8. A plant comprising the polypeptide of claim 4 .
9. A method for controlling a pest population comprising contacting said population with a pesticidally effective amount of the polypeptide of claim 1 .
10. An isolated nucleic acid that encodes a polypeptide of claim 1 .
11. An isolated nucleic acid that encodes a polypeptide of claim 2 .
12. An isolated nucleic acid that encodes a polypeptide of claim 3 .
13. An isolated nucleic acid that encodes a polypeptide of claim 4 .
14. The isolated nucleic acid of claim 10 having a sequence of SEQ ID NO: 1 or SEQ ID NO:3.
15. The polypeptide of claim 1 comprising an amino acid sequence of SEQ ID NO: 2 or SEQ ID NO:5.
16. A DNA construct comprising the nucleotide sequence of claim 10 operably linked to a promoter that is not derived from Bacillus thuringiensis and is capable of driving expression in a plant.
17. A transgenic plant that comprises the DNA construct of claim 16 stably incorporated into its genome.
18. A method for protecting a plant from a pest comprising introducing into said plant the construct of claim 16 .
19. The polypeptide of claim 1 , claim 2 , claim 3 , or claim 4 , wherein said polypeptide has insecticidal activity against corn rootworm.