Tissue matrices comprising placental stem cells
View Patent ↗A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
1. A tissue matrix comprising isolated CD34 − , CD10 + , SH2 + placental stem cells, wherein said tissue matrix is synthetic.
2. The tissue matrix of claim 1 wherein said placental stem cells are additionally CD90 + or CD45 − .
3. The tissue matrix of claim 2 wherein said placental stem cells are additionally CD90 + and CD45 − .
4. The tissue matrix of claim 1 wherein said placental stem cells are additionally one or more of CD29 + , CD38 − , CD44 + , CD54 + , SH3 + or SH4 + .
5. The tissue matrix of claim 4 wherein said placental stem cells are additionally one or more of CD29 + , CD38 − , CD44 + , CD54 + , SH3 + or SH4 + .
6. The tissue matrix of claim 4 wherein said placental stem cells are CD44 + .
7. The tissue matrix of claim 5 wherein said placental stem cells are CD44 + .
8. The tissue matrix of claim 1 , wherein said placental stem cells are additionally MHC Class II − .
9. The tissue matrix of claim 1 wherein said placental stem cells are tissue culture plastic adherent.
10. The tissue matrix of claim 1 , wherein said placental stem cells are additionally one or more of CD29 + , CD45 − , CD90 + , SH3 + , SH4 + , or MHC Class II − .
11. The tissue matrix of claim 10 , wherein said placental stem cells are additionally each of CD29 + , CD45 − , CD90 + , SH3 + , SH4 + , and MHC Class II − .
12. A method of making a tissue matrix, comprising seeding isolated CD34 − , CD 10 + , SH2 + placental stem cells onto a synthetic tissue matrix, wherein said plcental stem cells are from a human placenta that has been drained of cord blood.
13. The method of claim 12 wherein said placental stem cells are additionally CD90 + or CD45 − .
14. The method of claim 13 wherein said placental stem cells are additionally CD90 + and CD45 − .
15. The method of claim 12 wherein said placental stem cells are additionally one or more of CD29 + , CD38 − , CD44 + , CD54 + , SH3 + or SH4 + .
16. The method of claim 13 wherein said placental stem cells are additionally one or more of CD29 + , CD38 − , CD44 + , CD54 + , SH3 + or SH4 + .
17. The method of claim 13 wherein said placental stem cells are CD44 + .
18. The method of claim 14 wherein said placental stem cells are CD44 + .
19. The method of claim 12 , wherein said placental stem cells are additionally MHC Class II − .
20. The method of claim 12 wherein said placental stem cells are tissue culture plastic adherent.
21. The method of claim 12 , wherein said placental stem cells are additionally one or more of CD29 + , CD45 − , CD90 + , SH3 + , SH4 + , or MHC Class II − .
22. The method of claim 21 , wherein said placental stem cells are additionally each of CD29 + , CD45 − , CD90 + , SH3 + , SH4 + , and MHC Class II − .