Compositions and methods for detecting hepatitis B virus
View Patent ↗Compositions, methods and kits for detecting viral nucleic acids. Targets that can be detected in accordance with the invention include HBV and/or HIV-1 and/or HCV nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers that facilitate detection of very low levels of HBV nucleic acids.
1. A composition of oligonucleotides for amplifying and detecting an HBV nucleic acid wherein said composition comprises at least two amplification oligomers configured to generate an HBV amplicon from an HBV nucleic acid present in a test sample at a concentration as low as 200 copies/ml of reaction mixture, wherein:
(a) the first amplification oligomer consists of a target hybridizing sequence, optionally, an upstream sequence that is not complementary to an HBV target sequence, and a 5′ promoter sequence for an RNA polymerase, wherein the target hybridizing sequence consists of SEQ ID NO:25 or SEQ ID NO:28; and
(b) the second amplification oligomer consists of a target hybridizing sequence consisting of SEQ ID NO:15.
2. The composition of claim 1 , wherein the amplification oligomer at (a) is selected from the group consisting of: SEQ ID NOS:42, 45, and combinations thereof.
3. The composition of claim 1 , wherein the composition is provided as a component of a kit for the detection of HBV nucleic acid.
4. The composition of claim 1 , wherein the composition is provided as a component of a kit for screening donated blood samples or plasma samples for the presence of HBV.
5. The composition of claim 4 , further comprising components for amplification and/or detection of HIV-1nucleic acids, HCV nucleic acids or both.
6. The composition of claim 1 , further comprising a detectably labeled probe complementary to an HBV amplicon.
7. The composition of claim 6 , wherein the detectably labeled probe comprises a fluorescent label.
8. The composition of claim 6 , wherein said detectably labeled probe is a molecular beacon.
9. A method for screening donated blood or blood products for the presence of HBV, comprising the steps of:
a. contacting a test sample with
(i) an amplification oligomer, consisting of a target hybridizing sequence, optionally, an upstream sequence that is not complementary to an HBV target sequence, and a 5′ promoter sequence for an RNA polymerase, wherein the target hybridizing sequence consists of SEQ ID NO:25 or SEQ ID NO:28; and
(ii) an amplification oligomer consisting of a target hybridizing sequence consisting of SEQ ID NO:15; and
b. amplifying any HBV nucleic acid that is present in the test sample in an isothermal in vitro nucleic acid amplification reaction to generate an HBV amplicon; and
c. detecting the HBV amplicon; wherein detecting the presence of HBV in the test sample indicates that the blood product or the donated blood contains HBV nucleic acids, and wherein said method is capable of detecting HBV nucleic acid at a concentration at least as low as 200 copies/ml of reaction mixture.
10. The method of claim 9 , further comprising a separating step to separate HBV nucleic acids from one or more other components in a biological sample.
11. The method of claim 10 , wherein the separating step is performed using a target capture oligomer that includes a first sequence complementary to an HBV sequence and includes a second sequence for immobilization to a solid support.
12. The method of claim 9 , wherein the amplification oligomer at a.(i) consists of deoxyribonucleic acid residues, or the amplification oligomer at a.(ii) consists of deoxyribonucleic acid residues, or a combination thereof.
13. The method of claim 9 , wherein the isothermal in vitro amplification is a real-time amplification.
14. The method of claim 9 , wherein steps b. and c. are performed simultaneously.
15. The method of claim 9 , wherein the amplification oligomer at a.(i) is selected from the group consisting of: SEQ ID NOS:42, 45, and combinations thereof.
16. The method of claim 9 , wherein step c. is performed using a detection probe oligomer.
17. The method of claim 16 , wherein the detection probe oligomer is labeled with a chemiluminescent compound, a fluorescent compound, a quencher compound or a combination thereof.
18. The method of claim 9 , wherein the test sample is derived from a pool of blood donated by two or more blood donors.
19. The method of claim 9 , wherein the isothermal in vitro nucleic acid amplification reaction is a multiplex nucleic acid amplification reaction that amplifies HIV-1nucleic acid, HCV nucleic acid, or HIV-1nucleic acid and HCV nucleic acid in addition to HBV nucleic acid.
20. The method of claim 9 , wherein the detecting step comprises detecting HBV genotype G amplicons.
21. A method for screening plasma for the presence of HBV, comprising the steps of:
a. contacting a test sample with
(i) an amplification oligomer consisting of a target hybridizing sequence, optionally, an upstream sequence that is not complementary to an HBV target sequence, and a 5′ promoter sequence for an RNA polymerase, wherein the target hybridizing sequence consists of SEQ ID NO:25 or SEQ ID NO:28; and
(ii) an amplification oligomer consisting of a target hybridizing sequence consisting of SEQ ID NO:15; and
b. amplifying any HBV nucleic acid that is present in the test sample in an isothermal in vitro nucleic acid amplification reaction to generate an HBV amplicon; and
c. detecting the HBV amplicon;
wherein detecting the presence of HBV in the test sample indicates that the plasma contains HBV nucleic acids, and wherein said method is capable of detecting HBV nucleic acid at a concentration at least as low as 200 copies/ml of reaction mixture.
22. The method of claim 21 , further comprising a separating step to separate HBV nucleic acids from one or more other components in a biological sample.
23. The method of claim 22 , wherein the separating step is performed using a target capture oligomer that includes a first sequence complementary to an HBV sequence and includes a second sequence for immobilization to a solid support.
24. The method of claim 21 , wherein the amplification oligomer at a.(i) consists of deoxyribonucleic acid residues, or the amplification oligomer at a.(ii) consists of deoxyribonucleic acid residues, or a combination thereof.
25. The method of claim 21 , wherein the isothermal in vitro amplification is a real-time amplification.
26. The method of claim 21 , wherein steps b. and c. are performed simultaneously.
27. The method of claim 21 , wherein the amplification oligomer at a.(i) is selected from the group consisting of: SEQ ID NOS: 42, 45, and combinations thereof.
28. The method of claim 21 , wherein step c. is performed using a detection probe oligomer.
29. The method of claim 28 , wherein the detection probe oligomer is labeled with a chemiluminescent compound, a fluorescent compound, a quencher compound or a combination thereof.
30. The method of claim 21 , wherein the test sample is further tested for the presence of HIV-1nucleic acid, HCV nucleic acid or both.
31. The method of claim 21 , wherein the isothermal in vitro nucleic acid amplification reaction is a multiplex nucleic acid amplification reaction that amplifies HIV-1nucleic acid, HCV nucleic acid, or HIV-1nucleic acid and HCV nucleic acid in addition to HBV nucleic acid.
32. The method of claim 21 , wherein the detecting step comprises detecting HBV genotype G amplicons.
33. The method of claim 21 , wherein the test sample is derived from a pool of plasma donated by two or more donors.