Multiple-input analytical system
The present disclosure provides an automated sample processing system that can receive samples in different first and second formats and process both sample formats. The disclosure also provides a human papillomavirus testing apparatus. The apparatus has a first input to receive first test specimens in the form of pre-processed cervical samples, and a second input to receive unprocessed cervical samples. A first subsystem prepares second test specimens from the unprocessed cervical samples, and a second subsystem selectively processes and tests first specimens, second test specimens, or first and second test specimens to determine the presence of one or more human papillomavirus indicators.
1. An automated sample processing method, comprising:
providing first samples to an analytical system housed in a self-contained unit, the first samples provided in a first format in a first sample receptacle adapted to receive said first samples in said first format;
providing second samples to the analytical system, the second samples provided in a second format in a second sample receptacle adapted to receive said second samples in said second format, the second samples being of a different source than the first samples and the second format being different from the first format with respect to at least one of: a type of container in which said first samples and said second samples are contained, a collection media in which said first samples and said second samples are provided, and a processing stage at which said first samples and said second samples are provided;
processing said first samples into said second format within the self-contained unit using a first subsystem;
processing said second samples or said first samples in said second format within the self-contained unit using a second subsystem adapted to selectively process specimens in said second format.
2. The method of claim 1 , wherein said processing performed by said second subsystem determines the presence of one or more human papillomavirus DNAs.
3. The method of claim 1 , wherein said second format comprises isolated cells.
4. The method of claim 3 , wherein said processing performed by said first subsystem comprises isolation of nucleic acids from said isolated cells.
5. The method of claim 4 , wherein said processing performed by said second subsystem detects one or more HPV DNAs and comprises the steps of:
adding an RNA probe to a sample in said second format, thereby permitting formation of an RNA-DNA hybrid comprising probe RNA and HPV DNA contained in said sample, wherein said RNA probe comprises one or more HPV genomic sequences or a subsequences thereof;
capturing said RNA-DNA hybrid using an RNA-DNA hybrid-specific antibody bound to a plurality of capture beads;
washing said plurality of capture beads;
contacting said captured RNA-DNA hybrid with a labeled RNA-DNA hybrid-specific antibody, wherein said label produces a detectable signal that indicates the presence or absence of said RNA-DNA hybrid; and
detecting said detectable signal, thereby detecting the presence of one or more HPV DNAs.