Tissue implants for implantation and methods for preparing the same
View Patent ↗A method is provided for preparing a tissue implant for implantation. The method includes harvesting a tissue material from a human or an animal donor, treating the tissue material in a nuclease-containing solution, and thereafter treating the tissue material with an alkaline alcohol solution. The nuclease-containing solution includes an antimicrobial. The alkaline alcohol solution comprises sodium hydroxide and ethanol.
1. A method of preparing a tissue implant for implantation comprising:
treating a tissue material in a nuclease-containing solution, the nuclease-containing solution comprising an antibiotic-free antimicrobial, wherein the antibiotic-free antimicrobial comprises benzyl alcohol and the benzyl alcohol is present in the nuclease-containing solution in a concentration of 0.8 to about 2.0% v/v; and
treating the tissue material with an alkaline alcohol solution.
2. The method of claim 1 , wherein the nuclease-containing solution comprises DNase or RNase or a combination thereof.
3. The method of claim 1 , wherein the alkaline alcohol solution comprises ethanol.
4. The method of claim 1 , wherein the alkaline alcohol solution comprises sodium hydroxide.
5. A method of preparing a tissue implant for implantation comprising:
treating a tissue material in a nuclease-containing solution, the nuclease-containing solution comprising an antibiotic-free antimicrobial; and
treating the tissue material with an alkaline alcohol solution comprising ethanol, wherein the ethanol is present in a concentration of about 72 to about 88 percent v/v.
6. A method of preparing a tissue implant for implantation comprising:
treating a tissue material in a nuclease-containing solution, the nuclease-containing solution comprising an antibiotic-free antimicrobial; and
treating the tissue material with an alkaline alcohol solution comprising sodium hydroxide, wherein the alkaline alcohol solution is about 0.016 M to about 0.022 M sodium hydroxide.
7. A method of preparing a tissue implant for implantation comprising:
treating a tissue material in a nuclease-containing solution, the nuclease-containing solution comprising benzyl alcohol, which is present in the nuclease-containing solution in a concentration of 0.8 to about 2.0% v/v.
8. The method of claim 7 , wherein the nuclease-containing solution comprises DNase or RNase or a combination thereof.
9. A method of preparing a tissue implant for implantation comprising:
harvesting a tissue material from a human or an animal donor;
treating the tissue material in a nuclease-containing solution, the nuclease-containing solution comprising an antibiotic-free antimicrobial; and thereafter
treating the tissue material with an alkaline alcohol solution, which comprises sodium hydroxide and ethanol, wherein the alkaline alcohol comprises ethanol in a concentration of about 72 to about 88 percent v/v and 0.016 M to about 0.022 M sodium hydroxide.
10. The method of claim 9 , wherein the tissue material comprises human, bovine, or equine pericardium.
11. The method of claim 9 , wherein the antibiotic-free antimicrobial comprises benzyl alcohol.
12. A method of preparing a tissue implant for implantation comprising:
harvesting a tissue material from a human or an animal donor;
treating the tissue with a hypotonic solution to initiate cellular lysis;
treating the tissue material in a nuclease-containing solution, the nuclease-containing solution comprising benzyl alcohol present in the nuclease-containing solution in a concentration of 0.8 to about 2.0% v/v;
treating the tissue material with an alkaline alcohol solution comprising ethanol and sodium hydroxide, wherein the alkaline alcohol comprises ethanol in a concentration of about 72 to about 88 percent v/v and 0.016 M to about 0.022 M sodium hydroxide;
treating the tissue material with chlorine dioxide;
treating the tissue material with an antioxidant;
vacuum-sealing the tissue material in a package; and
irradiating the vacuum-sealed tissue material.