Silk fibroin materials and use thereof
The present application provides a composition comprising porous silk fibroin scaffold material. The porous silk fibroin scaffold can be used for tissue engineering. The porosity of the silk fibroin scaffold described herein can be adjusted to mimic the gradient of densities found in natural tissue.
1. A porous silk fibroin material comprising a three-dimensional silk fibroin body having interconnected pores, wherein the pores have a diameter of 155 to 1000 microns, wherein the material has a compressive modulus of at least 100 kPa.
2. The porous silk fibroin material of claim 1 , wherein the pores have a diameter of 155 to 500 microns.
3. The porous silk fibroin material of claim 1 , wherein the material has a compressive modulus of at least 150 kPa.
4. The porous silk fibroin material of claim 1 , wherein the material has a compressive modulus of at least 200 kPa.
5. The porous silk fibroin material of claim 1 , wherein the material has a compressive modulus of at least 250 kPa.
6. The porous silk fibroin material of claim 1 , wherein the material has a porosity above 80%.
7. The porous silk fibroin material of claim 1 , further comprising at least one additive.
8. The porous silk fibroin material of claim 7 , wherein the additive is a biologically active or pharmaceutically active compound.
9. The porous silk fibroin material of claim 7 , wherein at least one additive is a cell growth factor.
10. The porous silk fibroin material of claim 9 , wherein the cell growth factor is a cytokine.
11. The porous silk fibroin material of claim 7 , wherein at least one additive is a peptide that contains an integrin binding sequence.
12. The porous silk fibroin material of claim 7 , wherein at least one additive is a bone morphogenic protein, a basic fibroblast growth factor, an epidermal growth factor, a platelet-derived growth factor, an insulin-like growth factor or a transforming growth factor.
13. The porous silk fibroin material of claim 7 , wherein the additive is selected from peptides, antibodies, DNA, RNA, modified RNA/protein composites, glycogens or other sugars and alcohols.
14. The porous silk fibroin material of claim 7 , wherein the additive is selected from collagen, elastin, fibronectin, vitronectin, laminin and proteoglycans.
15. A porous silk fibroin material comprising a three-dimensional silk fibroin body having interconnected pores, wherein the pores have a mean pore size of 155±114 to 202±112 micrometers, and wherein the compressive modulus is 10±3 to 1000±75 kPa.
16. The porous silk fibroin material of claim 15 , wherein the material has a porosity above 80%.
17. The porous silk fibroin material of claim 15 , further comprising at least one additive.
18. The porous silk fibroin material of claim 17 , wherein at least one additive is a cell growth factor.
19. The porous silk fibroin material of claim 17 , wherein at least one additive is a peptide that contains an integrin binding sequence.