Polynucleotides encoding humanized anti-NGF antibodies
View Patent ↗Method for the humanization of the VH and VL variable regions of an animal antibody of known sequence, humanized animal antibody obtainable according to the method, in particular anti-NGF and anti-TrkA humanized animal antibodies.
1. A polynucleotide encoding:
(1) a humanized anti-NGF antibody that comprises:
(a) a VH region having the amino acid sequence set forth in SEQ ID NO:17 and
(b) a VL region having the amino acid sequence set forth in SEQ ID NO:18, or
(2) a fragment of the humanized anti-NGF antibody wherein said fragment binds NGF.
2. The polynucleotide of claim 1 , wherein the isotype of the humanized anti-NGF antibody encoded by the polynucleotide is IgG, IgM, IgA, IgD or IgE.
3. The polynucleotide of 2 , wherein the isotype of the humanized anti-NGF antibody encoded by the polynucleotide is IgG.
4. The polynucleotide of claim 1 , wherein the fragment of the humanized anti-NGF antibody encoded by the polynucleotide an Fab fragment, an (Fab′) 2 , an Fv fragment or an single chain Fv fragment (scFv).
5. The polynucleotide of claim 1 , wherein said antibody binds murine NGF.
6. An isolated cell comprising the polynucleotide of claim 1 .