Purification of factor XIII polypeptides from biological materials
The invention relates to a method for purifying a factor XIII polypeptide from a biological material, the method comprising subjecting the material to sequential chromatography on an anion-exchange matrix and a hydrophobic interaction matrix.
1. A method for purifying a factor XIII polypeptide from a biological material comprising subjecting the biological material to sequential chromatography on an anion-exchange matrix and a hydrophobic interaction matrix wherein no precipitation step to produce a factor XIII polypeptide-containing precipitate is performed, and wherein chromatography on an anion-exchange matrix is performed with a stabilizing agent selected from the group consisting of propylene glycol, propan-1,3-diol, propyl alcohol, and isopropyl alcohol.
2. A method according to claim 1 , wherein the factor XIII polypeptide is a recombinant factor XIII.
3. A method according to claim 2 , wherein the factor XIII polypeptide is human factor XIII.
4. A method according to claim 2 , wherein the factor XIII polypeptide is a dimer of A subunits.
5. A method according to claim 4 , wherein the factor XIII polypeptide is a dimer of human A subunits.
6. A method according to claim 1 , wherein the biological material is a biological fluid.
7. A method according to claim 6 , wherein the biological fluid is the supernatant of a cell lysate.
8. A method according to claim 7 , wherein the biological fluid is the supernatant of a yeast cell lysate.