Method for conducting maintenance on a chromatography column
View Patent ↗The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components.
1. A method for conducting maintenance on a chromatography column comprising:
a) providing a chromatography column comprising:
a dispersion system comprising a nozzle including a mobile phase pathway connected to a liquid inlet;
a tube with an adapter assembly and a base assembly, said adapter assembly moveable within a cavity of said tube in an operational mode;
the base assembly comprising a distributor and a bed support fastened to each other by releasable fixing means;
a collection system opposing the dispersion system; and
one or more seals;
b) releasing the tube from the base assembly;
c) lifting the tube and the adapter assembly above the base assembly to provide a gap for access therebetween;
d) unfastening the bed support from the distributor by releasing the fixing means;
e) removing the bed support from the column with a handling device;
f) conducting maintenance on the column and/or the bed support and/or said one or more seals;
g) returning the bed support to the column with said handling device and fastening the bed support to the distributor and the backing plate; and
h) lowering the tube and the adapter assembly and reconnecting the tube to the base assembly.
2. The method of claim 1 , further comprising removing the distributor from the column with the handling device and conducting maintenance on the column and/or said distributor.
3. The method of claim 1 , wherein said handling device is in the form of a cart having a pillar and comprising one or more arms projecting from said pillar for suspending the bed support or distributor thereon.
4. The method of claim 3 , wherein said arms are extendable from the pillar.
5. The method of claim 3 , wherein the arms are vertically movable on the pillar.
6. The method of claim 1 , wherein lifting the tube and the adapter assembly in step c) and/or lowering the tube and the adapter assembly in step h) is carried out by means of an overhead hoist or crane.
7. The method of claim 1 , wherein lifting the tube and the adapter assembly in step c) and/or lowering the tube and the adapter assembly in step h) is carried out by means of a drive system comprising one or more cylinders which are mounted to or are integral with the column.
8. The method of claim 1 , wherein removing the bed support from the column and/or returning the bed support to the column is carried out without substantially tilting the bed support.
9. The method of claim 1 , wherein removing the bed support from the column and/or returning the bed support to the column is carried out by tilting the bed support at an angle of more than 5° to the horizontal.