Canine leishmania vaccine
The present invention provides vectors that contain and express in vivo Leishmania KMP11 or an epitope thereof that elicits an immune response in a dog against Leishmania , compositions comprising said vectors, methods of vaccination against Leishmania , and kits for use with such methods and compositions.
1. A method of immunizing a subject susceptible to Leishmania infantum infection comprising a prime-boost administration regimen, wherein said regimen comprises a primo-administration of a composition comprising an expression vector comprising a polynucleotide encoding a Leishmania KMP 11 polypeptide having an amino acid sequence set forth as SEQ.ID.NO:4, followed by a boost administration of a vaccine comprising an expression vector comprising a polynucleotide encoding a Leishmania KMP 11polypeptide having an amino acid sequence set forth as SEQ.ID.NO:4, where in the composition induces an immune response.
2. The method of claim 1 , wherein the method comprises a primo-administration of a composition comprising an expression vector comprising a polynucleotide encoding a Leishmania KMP 11 polypeptide having an amino acid sequence set forth as SEQ.ID.NO:4, followed by a first boost administrations of a composition comprising an expression vector comprising a polynucleotide encoding a Leishmania KMP11 antigen having an amino acid sequence set forth as SEQ.ID.NO:4, and a second boost administrations of a composition comprising an expression vector comprising a polynucleotide encoding a Leishmania KMP 11 having an amino acid sequence set forth as SEQ.ID.NO:4.
3. The method of claim 2 , wherein the expression vector for the primo-administration is a plasmid, and wherein the expression vector for the first boost administration is a plasmid pVR1020 encoding a Leishmania KMP 11 polypeptide having an amino acid sequence set forth as SEQ.ID.NO:4, and wherein the expression vector for the second boost administration is a recombinant canary viral vector vCP2350 encoding a Leishmania KMP 11 polypeptide having an amino acid sequence set forth as SEQ.ID.NO:4.
4. The method of claim 1 , wherein the response induced is a humoral immune response, CD8 + T cell-mediated immune response and/or a CD4 + T cell-mediated immune response against KMP 11.
5. The method of claim 1 , wherein the polynucleotide is set forth as SEQ ID NO: 3.
6. The method of claim 1 , wherein the primo-administration is done intradermally with a needle free apparatus and the boost administration is done intramuscularly with a syringe and a needle and coupled to electrotransfer treatment.
7. The method of claim 1 , wherein the primo-administration is done intramuscularly with a syringe and a needle and coupled to electrotransfer treatment and the boost administration is done intradermally with a needle free apparatus.
8. The method of claim 2 , wherein the composition for the prime boost administration and the first boost administration comprise plasmids.