Carbonyl reductase, gene thereof and method of using the same
View Patent ↗The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea , a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
1. An isolated polypeptide selected from the group consisting of: (a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 4, and (b) a polypeptide having the amino acid sequence of SEQ ID NO: 4 except for at least one modification in SEQ ID NO: 4 selected from the group consisting of:
(1) the alanine at position 42 of SEQ ID NO: 4 is substituted by glycine,
(2) the glutamic acid at position 43 of SEQ ID NO: 4 is substituted by alanine,
(3) the lysine at position 46 of SEQ ID NO: 4 is substituted by glutamic acid, and
(4) the asparagine at position 49 of SEQ ID NO: 4 is substituted by lysine.
2. The isolated polypeptide of claim 1 , comprising all of the modifications (1) to (4).