RABBIT GENOME EDITING WITH ZINC FINGER NUCLEASES
The present invention provides a genetically modified rabbit or cell comprising at least one edited chromosomal sequence. In particular, the chromosomal sequence is edited using a zinc finger nuclease-mediated editing process. The disclosure also provides zinc finger nucleases that target specific chromosomal sequences in the rabbit genome.
1 . A genetically modified rabbit comprising at least one edited chromosomal sequence encoding a rabbit or human disease-related protein.
2 . The genetically modified rabbit of claim 1 , wherein the edited chromosomal sequence is inactivated, modified, or comprises an integrated sequence.
3 . The genetically modified rabbit of claim 1 , wherein the edited chromosomal sequence is inactivated such that no functional or even partially-functional rabbit or human disease-related protein is produced.
4 . The genetically modified rabbit of claim 3 , wherein inactivated chromosomal sequence comprises no exogenously introduced sequence(s).
5 . The genetically modified rabbit of claim 3 , further comprising at least one chromosomally integrated sequence encoding a functional rabbit or human disease-related protein.
6 . The genetically modified animal of claim 1 , wherein the rabbit or human disease is chosen from cardiovascular diseases; ocular disease; hypertriglyceridemia; altered fat metabolism; altered lipoprotein profile; liver defects; abnormal lipid metabolism; diabetes and obesity; autoimmune diseases; immunodeficiency and combinations thereof.
7 . The genetically modified rabbit of claim 1 , wherein the rabbit is heterozygous or homozygous for the at least one edited chromosomal sequence.
8 . The genetically modified rabbit of claim 1 , wherein the rabbit is an embryo, a juvenile, or an adult.
9 . The genetically modified rabbit of claim 1 , wherein the protein is a human disease-related protein.
10 . A rabbit embryo comprising at least one RNA molecule encoding a zinc finger nuclease that recognizes a chromosomal sequence encoding a rabbit or human disease-related protein, and, optionally, at least one donor polynucleotide comprising a sequence encoding a rabbit or human disease-related protein.
11 . The rabbit embryo of claim 10 , wherein the rabbit or human disease-related protein is chosen from cardiovascular diseases; ocular disease; hypertriglyceridemia; altered fat metabolism; altered lipoprotein profile; liver defects; abnormal lipid metabolism; diabetes and obesity; autoimmune diseases; immunodeficiency and combinations thereof.
12 . The rabbit embryo of claim 10 , wherein the protein is a human disease-related protein.
13 . A genetically modified rabbit cell, the cell comprising at least one edited chromosomal sequence encoding a rabbit or human disease-related protein.
14 . The genetically modified cell of claim 13 , wherein the edited chromosomal sequence is inactivated, modified, or comprises an integrated sequence.
15 . The genetically modified cell of claim 13 , wherein the edited chromosomal sequence is inactivated such that no functional rabbit or human disease-related protein is produced.
16 . The genetically modified cell of claim 15 , wherein the inactivated chromosomal sequence comprises no exogenously introduced sequence(s).
17 . The genetically modified cell of claim 16 , further comprising at least one chromosomally integrated sequence encoding a functional rabbit or human disease-related protein.
18 . The genetically modified cell of claim 13 , wherein the rabbit or human disease-related protein is chosen from cardiovascular diseases; ocular disease; hypertriglyceridemia; altered fat metabolism; altered lipoprotein profile; liver defects; abnormal lipid metabolism; diabetes and obesity; autoimmune diseases; immunodeficiency and combinations thereof.
19 . The genetically modified cell of claim 13 , wherein the cell is heterozygous or homozygous for the at least one edited chromosomal sequence.
20 . The genetically modified cell of claim 13 , wherein the protein is a human disease-related protein.
21 . A method for assessing the effect of an agent in a rabbit, the method comprising contacting a genetically modified rabbit comprising at least one edited chromosomal sequence encoding a rabbit or human disease-related protein with the agent, and comparing results of a selected parameter to results obtained from contacting a wild-type rabbit with the same agent, wherein the selected parameter is chosen from:
a) rate of elimination of the agent or its metabolite(s);
b) circulatory levels of the agent or its metabolite(s);
c) bioavailability of the agent or its metabolite(s);
d) rate of metabolism of the agent or its metabolite(s);
e) rate of clearance of the agent or its metabolite(s);
f) toxicity of the agent or its metabolite(s); and
g) efficacy of the agent or its metabolite(s).
22 . The method of claim 21 , wherein the agent is a pharmaceutically active ingredient, a drug, a toxin, biological active agent, or a chemical.
23 . The method of claim 21 , wherein the at least one edited chromosomal sequence is inactivated such that a functional rabbit or human disease-related protein is not produced, and wherein the animal further comprises at least one chromosomally integrated sequence encoding a functional rabbit or human disease-related protein.
24 . The method of claim 21 , wherein the rabbit or human disease is chosen from cardiovascular diseases; ocular disease; hypertriglyceridemia; altered fat metabolism; altered lipoprotein profile; liver defects; abnormal lipid metabolism; diabetes and obesity; autoimmune diseases; immunodeficiency and combinations thereof.
25 . The method of claim 21 , wherein the rabbit is one of the species chosen from New Zealand White rabbit, Dutch rabbit, Flemish Giant rabbit, European Rabbit ( Oryctolagus cuniculus ) and any other strains thereof; African Savanna Hare ( Lepus victoriae ); Alaskan Hare ( Lepus othus ); Amami Rabbit ( Pentalagus furnessi ); Antelope Jackrabbit ( Lepus alleni ); Arctic Hare ( Lepus arcticus ); Black Jackrabbit ( Lepus insularis ); Black-tailed Jackrabbit ( Lepus californicus ); Broom Hare ( Lepus castroviejoi ); Brush Rabbit ( Sylvilagus bachmani ); Bunyoro Rabbit ( Poelagus marjorita ); Burmese Hare ( Lepus pequensis ); Brown Hare ( Lepus capensis ); Chinese Hare ( Lepus sinensis ); Corsican Hare ( Lepus corsicanus ); Desert Cottontail ( Sylvilagus audubonii ); Dice's Cottontail ( Sylvilagus dicei ); Eastern Cottontail ( Sylvilagus floridanus ); Ethiopean Hare ( Lepus fagani ); Ethiopean Highland Hare ( Lepus starcki ); European Hare ( Lepus europaeus ); Granada Hare ( Lepus granatensis ); Hainan Hare ( Lepus hainanus ); Hispid Hare ( Caprolagus hispidus ); Indian Hare ( Lepus nigricollis ); Jameson's Red Rock Hare ( Pronolagus randensis ); Japanese Hare ( Lepus bracyurus ); Korean Hare ( Lepus coreanus ); Marsh Rabbit ( Sylvilagus palustris ); Mexican Cottontail ( Sylvilagus cunicularius ); Mountain Cottontail ( Sylvilagus nuttallii ); Mountain Hare ( Lepus timidus ); Natal Red Rock Hare ( Pronolagus crassicaudatus ); New England Cottontail ( Sylvilagus transitionalis ); Omilteme Cottontail ( Sylvilagus insonus ); Pygmy Rabbit ( Brachylagus idahoensis ); Riverine Rabbit ( Bunolagus monticularis ); San Jose Brush Rabbit ( Sylvilagus mansuetus ); Scrub Hare ( Lepus saxatilis ); Smith's Red Rock Hare ( Pronolagus rupestris ); Sumatra Short Eared Rabbit ( Nesolagus netscheri ); Snowshoe Hare ( Lepus americanus ); Swamp Rabbit ( Sylvilagus aquaticus ); Tapeti ( Sylvilagus brasiliensis ); Tehuantepec Jackrabbit ( Lepus flavigularis ); Tolai Hare ( Lepus tolai ); Tres Marias Cottontail ( Sylvilagus graysoni ); Volcano Rabbit ( Romerolagus diazi ); White-sided Jackrabbit ( Lepus callotis ); White-tailed Jackrabbit ( Lepus townsendii ); Woolly Hare ( Lepus oiostolus ); Yarkand Hare ( Lepus yarkandensis ); Yunnan Hare ( Lepus comus ) and other existing species.