Cell-image analyzing apparatus
View Patent ↗A cell-image analyzing apparatus is intended to analyze, using a cell image, a cell collective that forms a colony, and is provided with a computer. The cell-image analyzing apparatus has an image analysis software that makes the computer function as: a boundary element extracting means for extracting boundary elements of subjects upon analyzing the cell image; a possible colony region determining means for determining, as a possible colony region, a region surrounded by boundary elements of subjects and having a size greater than a first criterion value; and a colony region determining means for determining, in the possible colony region, a region containing more than a predetermined number of clustered regions each being surrounded by boundary elements of subjects and having a size smaller than a second criterion value, as a colony region.
1. A cell-image analyzing apparatus for analyzing a cell collective that forms a colony using a cell image, wherein the cell-image analyzing apparatus comprises a computer and image analysis software that controls the computer to function as units comprising:
a boundary element extracting unit for extracting boundary elements of subjects such as cells, intracellular organelles, or dust upon analyzing the cell image;
a possible colony region determining unit for determining, as a possible colony region, a region surrounded by boundary elements of subjects extracted by the boundary element extracting unit and having a size greater than a first criterion value; and
a colony region determining unit for determining a colony region, wherein the colony region determining unit divides the possible colony region determined by the possible colony region determining unit into the colony region and a non-colony region;
wherein the colony region determining unit is configured to predict that, upon growing a marked region from an arbitrary origin on the boundary elements of the subjects in the possible colony region by repeating a process of incorporating adjacent points into the marked region until the marked region contains a preset average number of boundary pixels for a single cell, the marked region containing the average number of boundary pixels is a region occupied by a single cell inside the possible colony region.
2. The cell-image analyzing apparatus according to claim 1 , wherein the colony region determining unit is further configured to distinguish, in the possible colony region, whether each region predicted as being occupied by a single cell belongs to a colony region or a non-colony region based on a morphologic characteristic.
3. The cell-image analyzing apparatus according to claim 2 , wherein the morphologic characteristic is circularity.
4. The cell-image analyzing apparatus according to claim 1 , wherein the colony region determining unit is further configured to determine, in the possible colony region, a region that contains more than a predetermined number of regions each of which is predicted as being occupied by a single cell and that has a size smaller than a second criterion value, as the colony region.