Protein showing enhanced expression in cancer cells
View Patent ↗A novel gene (designated 101P3A11 or PHOR-1) and its encoded protein, and variants thereof, are described wherein 101P3A11 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 101P3A11 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 101P3A11 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 101P3A11 can be used in active or passive immunization.
1. A method to identify a peptide that elicits an immune response to a protein comprising the amino acid sequence of SEQ ID NO: 28, which method comprises:
(a) identifying an HLA supertype for which binding of epitopes of said peptide is desired;
(b) selecting from the peptides listed for SEQ ID No: 28 in one of Tables V-XVIII and XXII-XLIX any peptides that comprise epitopes that bind alleles of said identified supertype;
(c) experimentally assessing the ability of said peptides to bind to at least one allele of said HLA supertype; and
(d) identifying a peptide that binds with an IC 50 equal to, or less than, 500 nanomolar to said HLA supertype allele.
2. The method of claim 1 , wherein peptides are selected that bind with an IC 50 equal to, or less than, 500 nanomolar to three alleles of said HLA supertype.