Methods and compositions for diagnosing and monitoring transplant rejection
View Patent ↗Methods of diagnosing or monitoring transplant rejection, particularly cardiac transplant rejection, in a patient by detecting the expression level of one or more genes in a patient, are described. Diagnostic oligonucleotides for diagnosing or monitoring transplant rejection, particularly cardiac transplant rejection and kits or systems containing the same are also described.
1. A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a nucleic acid in said patient to diagnose or monitor transplant rejection in said patient wherein said nucleic acid comprises the nucleotide sequence SEQ ID NO: 67.
2. The method of claim 1 wherein said transplant rejection is cardiac transplant rejection.
3. The method of claim 1 wherein said expression level is detected by measuring the RNA level expressed by said nucleic acid.
4. The method of claim 3 , further including isolating RNA from said patient prior to detecting said RNA level expressed by said nucleic acid.
5. The method of claim 3 wherein said RNA level is detected by PCR.
6. The method of claim 3 wherein said RNA level is detected by hybridization.
7. The method of claim 3 wherein said RNA level is detected by hybridization to an oligonucleotide.
8. The method of claim 7 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.