Plant capable of bearing seedless fruits and method of producing variety bearing seedless fruits
View Patent ↗Provided are a plant capable of bearing seedless fruits stably over several generations, a seedless fruit generated from this plant, a method of producing a variety capable of readily and reliably bearing seedless fruits, a variety produced by this production method, and a seedless fruit generated from the thus produced variety. Particularly provided is a plant capable of bearing seedless fruits, wherein the plant is obtained by a crossing method comprising a step (a) of selecting a first filial generation plant which is capable of bearing seedless fruits as well as having a male sterile trait and a parthenocarpic trait from the group of first filial generation plants generated by crossing between a plant of a male sterile line and a plant of a parthenocarpic line, and a step (b) of crossing the thus selected first filial generation plant with a plant of a fixed line which is capable of sustaining the parthenocarpic trait and the male sterile trait of the plant, as a pollen parent, to thereby generate a progeny plant having the parthenocarpic trait and the male sterile trait.
1. A method of producing a Capsicum annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing, wherein a C. annuum plant of a male sterile variety having a genetically determined parthenocarpic trait is backcrossed with a recurrent C. annuum plant of a fixed homozygous parthenocarpic line which is capable of bearing commercially acceptable seedless fruits and sustaining the genetically determined parthenocarpic trait and the genetically determined male sterile trait of the C. annuum plant, wherein said fixed line is used as a pollen parent and wherein the male sterile C. annuum plant is backcrossed to said fixed homozygous line as the recurrent parent at least one time,
to thereby produce a C. annuum variety bearing commercially acceptable seedless fruits stably over several generations.
2. The method of producing a C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing according to claim 1 , wherein said C. annuum variety is obtained by a crossing method comprising the following steps (a) and (b):
a step (a) of selecting a first filial generation plant which is capable of bearing seedless fruits as well as having a genetically determined male sterile trait and a genetically determined parthenocarpic trait from the group of first filial generation plants generated by crossing between a C. annuum pepper plant of a male sterile line and a C. annuum plant of a parthenocarpic line; and
a step (b) of backcrossing the thus selected first filial generation C. annuum plant with a C. annuum plant of a fixed homozygous parthenocarpic line which is capable of bearing commercially acceptable seedless fruits and sustaining the genetically determined parthenocarpic trait and the genetically determined male sterile trait of the selected first filial generation plant, wherein said fixed homozygous line is used as a pollen parent, to thereby generate a progeny C. annuum plant having the genetically determined parthenocarpic trait and the genetically determined male sterile trait and bearing commercially acceptable seedless fruits stably over several generations.
3. The method of producing a C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing according to claim 1 , wherein said C. annuum variety is obtained by a crossing method comprising the following steps (a) to (c) while repeating the step (c) at least one time:
a step (a) of selecting a first filial generation C. annuum plant which is capable of bearing seedless fruits as well as having a genetically determined male sterile trait and a genetically determined parthenocarpic trait from the group of first filial generation plants generated by crossing between a plant of a C. annuum male sterile line and a C. annuum plant of a parthenocarpic line;
a step (b) of crossing the thus selected first filial generation plant with a C. annuum plant of a fixed homozygous parthenocarpic line which is capable of bearing commercially acceptable seedless fruits and sustaining the genetically determined parthenocarpic trait and the genetically determined male sterile trait of the selected first filial generation plant, wherein said fixed line is used as a pollen parent, to thereby generate a progeny C. annuum plant having the genetically determined parthenocarpic trait and the genetically determined male sterile trait; and
a step (c) of backcrossing the thus generated progeny plant again with a C. annuum plant of the fixed homozygous parthenocarpic line used as the pollen parent in the step (b), wherein said fixed line is also used as a pollen parent, to thereby generate a progeny C. annuum plant having the genetically determined parthenocarpic trait and the genetically determined male sterile trait and bearing commercially acceptable seedless fruits stably over several generations.
4. The method of producing a C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing according to claim 1 , wherein said fixed homozygous parthenocarpic line is a line fixed through anther culture or ovule culture.
5. The method of producing a C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing according to claim 1 , wherein said variety capable of bearing commercially acceptable seedless fruits stably over several generations is a bell pepper.
6. The method of producing a C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing according to claim 5 , wherein said C. annuum variety is selected from the group consisting of 3 Mi 74-2 (FERM BP-10935), 3 Mi 74-4 (FERM BP-10936), and a progeny C. annuum plant of any of the preceding deposited plants;
wherein said progeny plant has the genetically determined parthenocarpic trait and the genetically determined male sterility trait and is capable of bearing commercially acceptable seedless fruits stably over several generations.
7. The method of producing a C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations by backcrossing according to claim 5 , wherein said fixed line is Mi 74 (FERM BP-10934).
8. A C. annuum variety capable of bearing commercially acceptable seedless fruits stably over several generations produced according to the method of claim 1 .
9. A commercially acceptable seedless fruit generated from a C. annuum plant of said variety according to claim 8 by means of fructification rather than fertilization.