Methods and compositions
The invention relates to a complex comprising a phage particle, said phage particle comprising (i) a polypeptide; (ii) a nucleic acid encoding the polypeptide of (i); (iii) a connector compound attached to said polypeptide wherein said connector compound is attached to the polypeptide by at least three discrete covalent bonds. The invention also relates to libraries, and to methods for making complexes and to methods of screening using same.
1. A method for making a complex, said method comprising (i) providing a phage particle comprising a polypeptide (ii) providing a tris-(bromomethyl)benzene (TBMB) connector compound at between 10 and 100 μM, and (iii) attaching said tris-(bromomethyl)benzene (TBMB) connector compound to said polypeptide by formation of at least three covalent bonds between said tris-(bromomethyl)benzene (TBMB) connector compound and said polypeptide.