Attachment of molecules to surfaces
View Patent ↗The present invention relates to methods, reagents, and substrates that can be used for, for example, immobilizing biomolecules, such as nucleic acids and proteins. In an embodiment, the present invention relates to surfaces coated with a polymer according to the present invention. In an embodiment, the present invention relates to methods for thermochemically and/or photochemically attaching molecules to a surface at a high density.
1. A substrate comprising a surface and a polymer, the polymer coating at least a portion of the surface;
the polymer comprising:
at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; and
one or more pendent reactive groups configured to form covalent bond with biomolecule, wherein one or more pendent reactive groups are selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof.
2. The substrate of claim 1 , wherein the polymer comprises:
N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof;
hydroxyalkylacrylate, hydroxyalkylmethacrylate, alkyl or aryl acrylate, alkyl or aryl methacrylate, ethoxyethoxyethylacrylate, polypropyleneglycolmonomethacrylate, or mixtures thereof;
vinylpyrrolidone, vinylcaprolactam, N-vinyl-N-methylacetamide, vinylmethylether, 2-vinylpyridine-N-oxide, vinylmethylsulfone, or mixtures thereof;
ethyleneglycol, ethyleneimine, PEG derivative of mono methacrylate, or mixtures thereof;
aminimide; or mixtures thereof.
3. A substrate comprising:
support surface; and
polymer coupled to the support surface; the polymer comprising:
at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; and
more than one reactive group selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof;
wherein the polymer is configured to form covalent bonds with biomolecule.
4. A composition comprising polymer;
the polymer comprising:
at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; and
one or more reactive groups configured to form covalent bonds with corresponding functional groups on biomolecule, wherein one or more pendent reactive groups are selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof;
the polymer being configured to be covalently attached to surface.
5. The composition of claim 4 , wherein the polymer comprises:
N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof;
hydroxyalkylacrylate, hydroxyalkylmethacrylate, alkyl or aryl acrylate, alkyl or aryl methacrylate, ethoxyethoxyethylacrylate, polypropyleneglycolmonomethacrylate, or mixtures thereof;
vinylpyrrolidone, vinylcaprolactam, N-vinyl-N-methylacetamide, vinylmethylether, 2-vinylpyridine-N-oxide, vinylmethylsulfone, or mixtures thereof;
ethyleneglycol, ethyleneimine, PEG derivative of monomethacrylate, or mixtures thereof;
aminimide; or
mixtures thereof
6. The composition of claim 4 , wherein the polymer coating comprises a monomer comprising an uncharged polar moiety other than primary amide in an amount effective to provide, when coated on a surface of a substrate, a contact angle of about 30° to about 60° for a sessile water drop.
7. A micro array comprising:
support surface;
polymer covalently coupled to the support surface;
the polymer comprising:
at least about 40 mol-% of monomer comprising an uncharged polar moiety other than primary amide; and
one or more reactive groups selected from thermochemically reactive groups, photo reactive groups, or mixtures thereof;
biomolecule covalently bound to the polymer in discrete spots.
8. The micro array of claim 7 , wherein the polymer comprises:
N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof;
hydroxyalkylacrylate, hydroxyalkylmethacrylate, alkyl or aryl acrylate, alkyl or aryl methacrylate, ethoxyethoxyethylacrylate, polypropyleneglycolmonomethacrylate, or mixtures thereof;
vinylpyrrolidone, vinylcaprolactam, N-vinyl-N-methylacetamide, vinylmethylether, 2-vinylpyridine-N-oxide, vinylmethylsulfone, or mixtures thereof;
ethyleneglycol, ethyleneimine, PEG derivative of monomethacrylate, or mixtures thereof;
aminimide; or
mixtures thereof.
9. The microarray of claim 7 , wherein the polymer coating comprises a monomer comprising an uncharged polar moiety other than primary amide in an amount effective to provide a contact angle of about 30° to about 60° for a sessile water drop.