Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
Aspects of the present invention relate to the discovery of a novel hepatitis C virus (HCV) isolate. Embodiments include HCV peptides, nucleic acids encoding said HCV peptides, antibodies directed to said peptides, compositions containing said nucleic acids and peptides, as well as methods of making and using the aforementioned compositions including, but not limited to, diagnostics and medicaments for the treatment and prevention of HCV infection.
1. An expression vector comprising:
a nucleic acid that comprises a nucleotide sequence encoding a hepatitis C virus (HCV) NS3/4A fusion protein that comprises a modified NS3/4A breakpoint at the proteolytic cleavage site between NS3 and NS4A, wherein said modified NS3/4A breakpoint comprises the amino acid sequence serine-serine-threonine.
2. The expression vector of claim 1 , wherein said nucleic acid is operatively associated with at least one regulatory element that directs expression of said nucleic acid in a human.
3. The expression vector of claim 1 , wherein said nucleotide sequence comprises at least 50 consecutive nucleotides of SEQ. ID. NO. 35.
4. An immunogenic composition comprising the expression vector of claim 1 and an adjuvant.
5. The expression vector of claim 1 , wherein said nucleotide sequence comprises at least 20 consecutive nucleotides of SEQ. ID. NO. 35.
6. The expression vector of claim 2 , wherein said nucleotide sequence comprises at least 70 consecutive nucleotides of SEQ. ID. NO. 35.
7. An immunogenic composition comprising the expression vector of claim 2 and an adjuvant.
8. The expression vector of claim 2 , wherein said nucleotide sequence comprises at least 20 consecutive nucleotides of SEQ. ID. NO. 35.
9. The immunogenic composition of claim 4 , wherein said nucleotide sequence comprises 50 consecutive nucleotides of SEQ. ID. NO. 35.
10. The immunogenic composition of claim 4 , wherein said nucleotide sequence comprises at least 20 consecutive nucleotides of SEQ. ID. NO. 35.
11. The immunogenic composition of claim 7 , wherein said nucleotide sequence comprises 70 consecutive nucleotides of SEQ. ID. NO. 35.
12. The immunogenic composition of claim 7 , wherein said nucleotide sequence comprises at least 20 consecutive nucleotides of SEQ. ID. NO. 35.