Inositol polyphosphate 2-kinase genes and uses thereof
View Patent ↗This invention relates to newly identified polynucleotides and polypeptides in the phytic acid biosynthetic pathways, variants and derivatives of the same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular, the invention relates to polynucleotides encoding inositol polyphosphate 2-kinase (IPP2-K) and polypeptides exhibiting such activity to modulate the phytic acid biosynthesis in such a way as to decrease phytic acid and/or increase the non-phytic acid phosphorous, especially in corn or soy animal feedstuffs.
1. A chimeric polynucleotide that encodes a plant inositol polyphosphate 2-kinase comprising a member selected from the group consisting of:
(a) a polynucleotide comprising SEQ ID NO: 1;
(b) a polynucleotide comprising at least 97% sequence identity to SEQ ID NO: 1, wherein the % sequence identity is based on the entire sequence of SEQ ID NO: 1 and is determined by GAP 10 analysis using default parameters and the Needleman and Wunsch algorithm; and,
(c) a polynucleotide encoding a polypeptide comprising SEQ ID NO: 2.
2. The polynucleotide of claim 1 , wherein the polynucleotide is DNA.
3. The polynucleotide of claim 1 , wherein the polynucleotide is RNA.
4. The polynucleotide of claim 1 , wherein the polynucleotide is a plant polynucleotide.
5. A chimeric polynucleotide that encodes an inositol polyphosphate 2-kinase having at least 98% sequence identity to SEQ ID NO: 1, wherein the % sequence identity is based on the entire sequence of SEQ ID NO: 1 and is determined by GAP 10 analysis using default parameters and the Needleman and Wunsch algorithm.
6. A chimeric polynucleotide that encodes SEQ ID NO:2.
7. The chimeric polynucleotide according to claim 6 encoding an inositol polyphosphate 2-kinase protein, wherein said polynucleotide hybridizes under stringent conditions with the full complement of SEQ ID NO: 1, and the stringent conditions include a wash step in 0.1×SSC at 60° C.
8. A chimeric polynucleotide that comprises a sequence of SEQ ID NO:1.
9. The polynucleotide of claim 1 , wherein the polynucleotide comprises a sequence of a nucleic acid amplified from plant nucleic acid using primers based on SEQ ID NO: 1.
10. The polynucleotide of claim 1 , wherein the inositol polyphosphate 2-kinase is a corn inositol polyphosphate 2-kinase.