COMPOSITIONS OF ADULT ORGAN STEM CELLS AND USES THEREOF
Compositions of isolated adult organ stem cells, including hematopoietic stem cells, cardiac stem cells, and kidney stem cells, are disclosed. In particular, the present invention provides c-kit positive, lineage negative adult stem cells that can be isolated from adult organ tissue. Such stem cells are capable of generating all the cell lineages of the organ tissue from which they were isolated. Methods for repairing damaged organ tissue with the isolated organ stem cells are also disclosed.
1 . A method of isolating resident adult stem cells from an adult organ comprising:
culturing a tissue specimen from said organ in culture, thereby forming a tissue explant;
selecting cells from the cultured explant that are c-kit positive, and
isolating said c-kit positive cells, wherein said isolated c-kit positive cells are resident adult stem cells.
2 . The method of claim 1 , wherein said isolated c-kit positive cells are lineage negative.
3 . The method of claim 1 , wherein said isolated c-kit positive cells are capable of generating one or more of the cell lineages of said adult organ.
4 . The method of claim 1 , further comprising expanding said isolated c-kit positive cells in culture.
5 . The method of claim 1 , wherein said adult organ is the heart, kidney, liver, spleen, pancreas, intestine, lung, stomach, brain, retina, esophagus, bladder, epidermis, or bone marrow.
6 . The method of claim 1 , wherein said isolated c-kit positive cells are clonogenic, multipotent, and self-renewing.
7 . A method of repairing and/or regenerating damaged tissue of an organ in a patient in need thereof comprising isolating c-kit positive stem cells from a tissue specimen of said organ and administering said isolated c-kit positive stem cells to the damaged tissue, wherein said c-kit positive stem cells generate differentiated cells that assemble into new organ tissue following their administration, thereby repairing and/or regenerating the damaged organ.
8 . The method of claim 7 , wherein said isolated c-kit positive stem cells are expanded in culture prior to administration to the damaged tissue.
9 . The method of claim 7 , wherein the c-kit positive stem cells are lineage negative.
10 . The method of claim 7 , wherein the c-kit positive stem cells are capable of generating one or more of the cell lineages of said adult organ.
11 . The method of claim 7 , wherein the organ is the heart, kidney, liver, spleen, pancreas, intestine, lung, stomach, brain, retina, esophagus, bladder, epidermis, or bone marrow.
12 . The method of claim 11 , wherein the organ is the kidney.
13 . The method of claim 12 , wherein the damaged tissue results from an acute kidney injury.
14 . The method of claim 7 , wherein the damaged tissue results from an ischemic event.
15 . The method of claim 7 , wherein the said isolated c-kit positive stem cells are activated by one or more cytokines prior to administration to the damaged tissue.
16 . The method of claim 7 , wherein the c-kit positive stem cells are autologous.
17 . A pharmaceutical composition comprising isolated adult organ stem cells and a pharmaceutically acceptable carrier, wherein said isolated adult organ stem cells are c-kit positive, lineage negative, and isolated from the tissue of an adult organ.
18 . The composition of claim 17 , wherein said organ is heart, kidney, liver, spleen, pancreas, intestine, lung, stomach, brain, retina, esophagus, bladder, epidermis, or bone marrow.
19 . The composition of claim 17 , wherein said isolated adult organ stem cells are capable of generating one or more of the cell lineages of said adult organ.