Human artificial chromosome (HAC) vector
View Patent ↗The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.
1. A human artificial chromosome vector comprising a fragment of human chromosome 21, wherein said human artificial chromosome vector possesses a whole long arm region from the centromere to AL163204, exists as a monomer and is stably transmitted as a single copy, wherein the fragment of chromosome 21 is about 2-16 Mb, wherein:
i) a distal region of the long arm of human chromosome 21 is deleted within the AL163204 region; or
ii) a distal region of the long arm of human chromosome 21 is deleted within the AL163204 region and a distal region of the short arm of human chromosome 21 is deleted at AL163201,
and wherein an expression cassette comprising a recognition site for a site-specific recombination enzyme is inserted into a proximal region of the long arm and/or a proximal region of the short arm of human chromosome 21.
2. The human artificial chromosome vector according to claim 1 , wherein the site-specific recombination enzyme is Cre enzyme.
3. The human artificial chromosome vector according to claim 1 or 2 , wherein the recognition site for the site-specific recombination enzyme is the loxP sequence.
4. The human artificial chromosome vector according to claim 1 , wherein the recognition site for the site-specific recombination enzyme is inserted into AL163203 in the proximal region of the long arm of human chromosome 21.
5. The human artificial chromosome vector according to claim 1 , wherein the deletion of the distal region of the long arm and/or the distal region of the short arm is by substitution with an artificial telomere sequence.