OLIGONUCLEOTIDES-TRANSFERRING PREPARATIONS
Preparations for transferring efficiently oligonucleotides necessary in antisense therapy or the like into animal cells so as to be useful in treatment for various diseases, which comprises a collagen as an essential component are provided.
1 . An article from which an oligonucleotide is released comprising an oligonucleotide of 5 to 30 nucleotides in length and a water soluble atelocollagen that has a degree of cross-linking of 3 or less.
2 . The article of claim 1 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
3 . The article of claim 1 , in which the atelocollagen is a water soluble type I atelocollagen.
4 . The article of claim 1 , in which the oligonucleotide has at least one phosphorothioate bond.
5 . The article of claim 1 , in which the oligonucleotide inhibits expression of a target mRNA.
6 . The article of claim 1 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
7 . The article of claim 1 , in which the oligonucleotide is a DNA or a DNA derivative.
8 . The article of claim 1 , in which the oligonucleotide is a RNA or a RNA derivative.
9 . A water soluble article from which an oligonucleotide is released comprising an oligonucleotide of 5 to 30 nucleotides in length and a water soluble type I atelocollagen.
10 . The article of claim 9 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
11 . The article of claim 9 , in which the oligonucleotide has at least one phosphorothioate bond.
12 . The article of claim 9 , in which the oligonucleotide inhibits translation of a target mRNA.
13 . The article of claim 9 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
14 . The article of claim 9 , in which the oligonucleotide is a DNA or a DNA derivative.
15 . The article of claim 9 , in which the oligonucleotide is a RNA or a RNA derivative.
16 . A solution comprising an oligonucleotide of 5 to 30 nucleotides in length and a water soluble atelocollagen that has a degree of cross-linking of 3 or less.
17 . The solution of claim 16 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
18 . The solution of claim 16 , in which the atelocollagen is a type I atelocollagen.
19 . The solution of claim 16 , in which the oligonucleotide has at least one phosphorothioate bond.
20 . The solution of claim 16 , in which the oligonucleotide inhibits expression of a target mRNA.
21 . The solution of claim 16 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
22 . The solution of claim 16 , in which the oligonucleotide is a DNA or a DNA derivative.
23 . The solution of claim 16 , in which the oligonucleotide is a RNA or a RNA derivative.
24 . A water soluble article from which an oligonucleotide is released consisting essentially of an oligonucleotide of 5 to 30 nucleotides in length and a water soluble atelocollagen that has a degree of cross-linking of 3 or less.
25 . The article of claim 24 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
26 . The article of claim 24 , in which the atelocollagen is a water soluble type I atelocollagen.
27 . The article of claim 24 , in which the oligonucleotide has at least one phosphorothioate bond.
28 . The article of claim 24 , in which the oligonucleotide inhibits expression of a target mRNA.
29 . The article of claim 24 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
30 . The article of claim 24 , in which the oligonucleotide is a DNA or a DNA derivative.
31 . The article of claim 24 , in which the oligonucleotide is a RNA or a RNA derivative.
32 . A water soluble article consisting essentially of an oligonucleotide of 5 to 30 nucleotides in length and a water soluble type I atelocollagen.
33 . The article of claim 32 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
34 . The article of claim 32 , in which the oligonucleotide has at least one phosphorothioate bond.
35 . The article of claim 32 , in which the oligonucleotide inhibits translation of a target mRNA.
36 . The article of claim 32 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
37 . The article of claim 32 , in which the oligonucleotide is a DNA or a DNA derivative.
38 . The article of claim 32 , in which the oligonucleotide is a RNA or a RNA derivative.