Corn event DAS-59122-7 and methods for detection thereof
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
1. An isolated DNA molecule comprising a junction sequence comprising a sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof.
2. An insect resistant corn plant, or part thereof, comprising corn event DAS-59122-7, wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof forms part of the plant's genome.
3. A descent plant of the insect resistant corn plant of claim 2 , wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof, forms part of the descent plant's genome, wherein said descent plant comprises corn event DAS-59122-7.
4. A seed of the plant of claim 2 , wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof, forms part of the seed's genome, wherein said seed comprises corn event DAS-59122-7.
5. A seed of the plant of claim 3 , wherein DNA having at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof, forms part of the seed's genome, wherein said seed comprises corn event DAS-59122-7.
6. A method of producing an insect resistant corn plant, said method comprising breeding with the plant of claim 2 , and selecting progeny by analyzing for at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof, wherein said progeny comprises corn event DAS-59122-7.
7. A method of producing an insect resistant corn plant, said method comprising breeding with the plant of claim 3 , and selecting progeny by analyzing for at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof, wherein said progeny comprises corn event DAS-59122-7.
8. A method of detecting event DAS-59122-7 in corn tissue, said method comprising:
(a) contacting a sample of said corn tissue with a polynucleotide probe that hybridizes under stringent hybridization conditions with one or more DNA sequences selected from the group consisting of SEQ ID NO: 33, 35, and complements thereof;
(b) subjecting said sample and probe to stringent hybridization conditions; and
(c) analyzing for hybridization of the probe.