Methods and compositions for monitoring and risk prediction in cardiorenal syndrome
View Patent ↗The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects. In particular, the invention relates to methods and compositions selected to monitor cardiorenal syndrome using assays that detect NGAL, preferably together with assays that detect natriuretic peptides such as BNP. Such methods and compositions can provide early indications of a deterioration in cardiorenal syndrome status, including prognosis regarding mortality and worsening renal function.
1. A method of assigning a risk of an adverse outcome to a patient suffering from heart failure, comprising:
performing two or more of the assay methods in steps (a)-(d) below:
a. an assay method that detects NGAL on a body fluid sample obtained from said patient, thereby providing an NGAL assay result;
b. an assay method that detects one or more of BNP, NT-proBNP and proBNP on a body fluid sample obtained from said patient, thereby providing a natriuretic peptide assay result;
c. an assay method that detects cystatin C on a body fluid sample obtained from said patient, thereby providing a cystatin C assay result;
d. an assay method that detects serum creatinine on a body fluid sample obtained from said patient, thereby providing a serum creatinine assay result; and
correlating the assay results obtained to said risk of an adverse outcome.
2. The method according to claim 1 , wherein said adverse outcome is mortality, congestive heart failure, or an acute coronary syndrome.
3. The method according to claim 1 , wherein,
if step (a) is performed, said correlating step comprises comparing said NGAL assay result to a baseline NGAL result, and relating the NGAL assay result to the patient's risk by assigning an increased likelihood of an adverse outcome to said patient when said NGAL assay result is greater than said baseline NGAL result, relative to a risk assigned when said NGAL assay result is less than said baseline NGAL result, or by assigning a decreased likelihood of an adverse outcome to said patient when said NGAL assay result is less than said baseline NGAL result, relative to a risk assigned when said NGAL assay result is greater than said baseline NGAL result; and
if step (b) is performed, said correlating step comprises comparing said natriuretic peptide assay result to a baseline natriuretic peptide result, and relating the natriuretic peptide assay result to the patient's mortality risk by assigning an increased likelihood of an adverse outcome to said patient when said natriuretic peptide assay result is greater than said baseline natriuretic peptide result, relative to a risk assigned when said natriuretic peptide assay result is less than said baseline natriuretic peptide result, or by assigning a decreased likelihood of an adverse outcome to said patient when said natriuretic peptide assay result is less than said baseline natriuretic peptide result, relative to a risk assigned when said natriuretic peptide assay result is greater than said baseline natriuretic peptide result; and
if step (c) is performed, said correlating step comprises comparing said cystatin C assay result to a baseline cystatin C result, and relating the cystatin C assay result to the patient's mortality risk by assigning an increased likelihood of an adverse outcome to said patient when said cystatin C assay result is greater than said baseline cystatin C result, relative to a risk assigned when said cystatin C assay result is less than said baseline cystatin C result, or by assigning a decreased likelihood of an adverse outcome to said patient when said cystatin C assay result is less than said baseline cystatin C result, relative to a risk assigned when said cystatin C assay result is greater than said baseline cystatin C result; and
if step (d) is performed, said correlating step comprises comparing said serum creatinine assay result to a baseline serum creatinine result, and relating the serum creatinine assay result to the patient's mortality risk by assigning an increased likelihood of an adverse outcome to said patient when said serum creatinine assay result is less than said baseline serum creatinine result, relative to a risk assigned when said serum creatinine assay result is greater than said baseline serum creatinine result, or by assigning a decreased likelihood of an adverse outcome to said patient when said serum creatinine assay result is greater than said baseline serum creatinine result, relative to a risk assigned when said serum creatinine assay result is less than said baseline serum creatinine result.