Quantitative multiplex detection of pathogen biomarkers
View Patent ↗The present invention addresses the simultaneous detection and quantitative measurement of multiple biomolecules, e.g., pathogen biomarkers through either a sandwich assay approach or a lipid insertion approach. The invention can further employ a multichannel, structure with multi-sensor elements per channel.
1. A chip for use in simultaneous detection and quantitative measurement of multiple biomolecules, the chip comprising: a substrate, a patterned waveguide upon said substrate, a film layer upon the patterned waveguide, a reference biomolecule including an attached inorganic fluorescent reporter molecule, the reference biomolecule attached to said film layer as an assay element standard, and an amount of at least two recognition ligands attached to said film layer for capture of at least two differing target biomolecules.
2. A multichannel structure for use in simultaneous detection and quantitative measurement of multiple biomolecules comprising:
a substrate having at least one fluid channel therein wherein each fluid channel has at least two sensing element regions per channel, said multichannel structure further including a light absorbing material situated around each individual sensing element region thereby essentially eliminating cross-talk coupling between sensing element regions, each sensing element region having: (a) a film layer upon the substrate and (b) a reference biomolecule including an attached inorganic fluorescent reporter molecule, the reference biomolecule attached to said film layer as an assay element standard; and,
at least one additional recognition ligand attached to said film layer for capture of a target biomolecule having at least two binding sites.