Mitochondrial enhancement of cells
View Patent ↗Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of stem cells. Certain embodiments disclosed herein include, but are not limited to, methods of modifying stem cells, or methods of administering modified stem cells to at least one biological tissue.
1. A method of modifying a eukaryotic cell, comprising: providing at least one exogenous cellular component derived from a maternally genetically related cell, further comprising measuring the membrane potential of the eukaryotic cell or the maternally genetically related cell at least one of prior to, during, or subsequent to providing the at least one exogenous cellular component to the eukaryotic cell, wherein the exogenous cellular component is an exogenous mitochondria.
2. The method of claim 1 , wherein at least one of the exogenous mitochondria, exogenous mitochondrial DNA, or exogenous cell nucleus, is provided to at least one intracellular compartment of the eukaryotic cell.
3. The method of claim 1 , wherein at least one exogenous cellular component is provided in response to the presence or level of at least one eukaryotic cell indicator.
4. The method of claim 3 , wherein the at least one eukaryotic cell indicator includes at least one indicator of one or more of a property of the eukaryotic cell; a property of administering the at least one mitochondria, mitochondrial DNA, or cell nucleus, to the eukaryotic cell; eukaryotic cell death; eukaryotic cell division; eukaryotic cell cytoskeletal rearrangement; eukaryotic cell mitochondrial quality, quantity, or arrangement; or eukaryotic cell or tissue secretion.
5. The method of claim 4 , wherein the property of the eukaryotic cell includes at least one of eukaryotic cell size, eukaryotic cell stage, eukaryotic cell quality, health of the subject from which the eukaryotic cell originates, species of the subject from which the eukaryotic cell originates, immunological background of the eukaryotic cell, mitotic rate of the eukaryotic cell, metabolic profile of the eukaryotic cell, genomic profile of the eukaryotic cell, transcriptomic profile of the eukaryotic cell, or proteomic profile of the eukaryotic cell, or storage conditions of the eukaryotic cell.
6. The method of claim 5 , wherein the storage conditions of the eukaryotic cell include at least one of duration of storage time, storage temperature, storage size, eukaryotic cell dilution, or storage solution(s).
7. The method of claim 1 , further comprising selecting the eukaryotic cell for further manipulation.
8. A method of modifying a eukaryotic cell, comprising: providing at least one exogenous cellular component derived from a maternally genetically related cell, further comprising measuring the membrane potential of the eukaryotic cell or the maternally genetically related cell at least one of prior to, during, or subsequent to providing the at least one exogenous cellular component to the eukaryotic cell, wherein the maternally genetically related cell is derived from the child of the source of the eukaryotic cell, wherein the source of the eukaryotic cell is female, and wherein the exogenous cellular component is an exogenous mitochondria.
9. The method of claim 8 , wherein the maternally genetically related cell includes or is derived from at least one of an oocyte, or stem cell from the child of the source of the eukaryotic cell.
10. A method of modifying a eukaryotic cell, comprising: providing at least one exogenous cellular component derived from a maternally genetically related cell, further comprising measuring the membrane potential of the eukaryotic cell or the maternally genetically related cell at least one of prior to, during, or subsequent to providing the at least one exogenous cellular component to the eukaryotic cell, wherein the source of the exogenous cellular component is of chronologically younger age than the source of the eukaryotic cell, wherein the exogenous cellular component is an exogenous mitochondria.