Microscope apparatus having a modulation-region adjusting unit that moves a wavefront modulation region in response to pivoting of mirrors
View Patent ↗Illuminating light is two-dimensionally scanned without changing the ability to focus illuminating light on a specimen. A microscope has a spatial light modulator for the wavefront of illuminating light from a light source; a scanner having two mirrors independently pivoted about two non-parallel axes; a relay optical system guiding the illuminating light, whose traveling direction has been changed by the scanner, to an objective optical system; and an adjusting unit that moves a wavefront modulation region of the modulator, in which an image is formed, in response to pivoting of the mirrors, such that an image at the pupil position of the objective optical system assuming that the mirrors are stopped is moved opposite to the direction of movement of the image relayed to the pupil position of the objective optical system assuming that the mirrors are pivoting while the image on the spatial light modulator is fixed.
1. A microscope apparatus comprising:
a spatial light modulator that modulates the wavefront of illuminating light from a light source;
a scanner that has two mirrors independently pivoted about two non-parallel axes, and that two-dimensionally scans the illuminating light whose wavefront has been modulated by the spatial light modulator;
a relay optical system that guides the illuminating light, whose traveling direction has been changed by the scanner, to an objective optical system; and
a modulation-region adjusting unit that moves a wavefront modulation region of the spatial light modulator, in which an image is formed, in response to pivoting of the mirrors,
wherein:
a first direction is a direction in which the image of the modulation region relayed to a pupil position of the objective optical system would move when the mirrors are pivoted while the modulation region is fixed,
a second direction is a direction in which the image of the modulation region relayed to the pupil position of the objective optical system would move when the mirrors are stationary while the modulation region is moved, and
the modulation-region adjusting unit moves the modulation region when the mirrors are pivoted so that the second direction is opposite to the first direction, thereby keeping a position of the image of the modulation region relayed to the pupil position of the objective optical system stationary.
2. The microscope apparatus according to claim 1 , wherein the modulation-region adjusting unit moves the modulation region in the spatial light modulator.
3. The microscope apparatus according to claim 1 , wherein the modulation-region adjusting unit moves the spatial light modulator.
4. The microscope apparatus according to claim 1 , wherein the pivot axis of one mirror is disposed in a plane perpendicular to the pivot axis of the other mirror of the scanner, and the pupil position of the objective optical system and the position of one of the mirrors on the pivot axis have an optically conjugate relationship.
5. The microscope apparatus according to claim 4 , wherein the pupil position of the objective optical system and the position, on the pivot axis, of the mirror that is pivoted at a higher speed in the two mirrors have an optically conjugate relationship.
6. The microscope apparatus according to claim 1 , wherein the pupil position of the objective optical system and a position between the two mirrors have an optically conjugate relationship.
7. The microscope apparatus according to claim 1 , wherein the relay optical system is a second relay optical system, and the microscope apparatus includes a first relay optical system that relays an image on the spatial light modulator to a position having an optically conjugate relationship with the pupil position of the objective optical system of the second relay optical system.
8. The microscope apparatus according to claim 1 , wherein the modulation-region adjusting unit is a control unit that pivots the mirror.
9. The microscope apparatus according to claim 8 , wherein the control unit outputs angle instruction signals instructing pivot angles to motors that pivot the mirrors of the scanner.