Thrombin activator compositions and methods of making and using the same
View Patent ↗Disclosed are compositions for activating thrombin precursors to thrombin. The compositions provided include polypeptide compositions wherein the pre-pro-sequence comprises a thrombin cleavage site. The compositions provided also include polynucleotides encoding said polypeptides and recombinant systems for expressing said polypeptides. This disclosure also relates to methods for producing said compositions, recovering said compositions, activating said compositions purifying said compositions and producing active thrombin molecules using the active form of said compositions.
1. A recombinant metalloprotease pre-pro-activator comprising, from an amino-terminal position to a carboxyl-terminal position, a pre-pro leader that shares at least 60% sequence identity with the pre-pro leader from Echis carinatus ecarin wild-type metalloprotease pre-pro-activator; a thrombin cleavage site consisting of a glycine, a proline, and an arginine; and a mature activator that shares at least 60% sequence identity with the mature activator from the Echis carinatus ecarin wild-type metalloprotease pre-pro-activator.
2. The recombinant metalloprotease pre-pro-activator of claim 1 , wherein the pre-pro leader shares at least 60% sequence identity with amino acid residues 1-187 of SEQ ID NO:100; and wherein the mature activator shares at least 60% sequence identity with amino acid residues 191-616 of SEQ ID NO:100.
3. The recombinant metalloprotease pre-pro-activator of claim 1 , wherein said pre-pro-activator shares at least 90% sequence identity with the amino acid sequence of residues 1-616 of SEQ ID NO:2.
4. The recombinant metalloprotease pre-pro-activator of claim 1 , wherein said pre-pro-activator shares at least 99% sequence identity with the amino acid sequence of residues 1-616 of SEQ ID NO:2.
5. The recombinant metalloprotease pre-pro-activator as in claim 1 , further comprising an affinity tag positioned carboxyl-terminal to the mature activator.
6. The recombinant metalloprotease pre-pro-activator of claim 5 , wherein said affinity tag is a histidine tag.
7. The recombinant metalloprotease pre-pro-activator as in claim 1 , wherein said pre-pro-activator consists essentially of the pre-pro leader, the thrombin cleavage site, and the mature activator.
8. The recombinant metalloprotease pre-pro-activator as in claim 1 , wherein said pre-pro leader comprises at least thirty-five contiguous amino acid residues from amino acid residues 1-187 of SEQ ID NO:100.
9. The recombinant metalloprotease pre-pro-activator as in claim 1 , wherein said pre-pro leader comprises amino acid residues 153-187 of SEQ ID NO:100.
10. The recombinant metalloprotease pre-pro-activator as in claim 1 , wherein said pre-pro leader comprises amino acid residues 1-187 of SEQ ID NO:100.
11. A recombinant metalloprotease pre-pro-activator comprising the amino acid sequence of residues 1-616 of SEQ ID NO:2.
12. An isolated pre-pro-activator polypeptide produced by transfecting a host cell with an expression vector comprising a polynucleotide sequence encoding a pre-pro-activator as in claim 1 ; and expressing the encoded pre-pro-activator from said expression vector.
13. The recombinant metalloprotease pre-pro-activator of claim 1 , wherein said mature activator comprises amino acid residues 188-616 of SEQ ID NO:2, 189-616 of SEQ ID NO:2, 190-616 of SEQ ID NO:2, or 191-616 of SEQ ID NO:2.