Gradient-elution multi-column separation method
The invention relates to a method for separating fractions of a mixture to be separated including the steps of providing a device comprising at least two chromatography columns mounted in series, discontinuously injecting a mixture to be separated, in a single point of the device, discontinuously drawing off the fraction enriched in the product of interest, in a single point of the device, and injecting eluents in each column and displacing the points for injection eluents, the eluents having a different eluting power. The method allows efficient separation which is simple to perform.
1. A method for separating fractions of a mixture to be separated comprising the steps of
providing a device comprising at least two chromatography columns mounted in series,
discontinuously injecting a mixture to be separated, in a first single point of the device,
discontinuously drawing off the fraction enriched in the product of interest, in a second single point of the device, and
injecting eluents in a plurality of points of each column and displacing the plurality of points for injecting eluents, the eluents having a different eluting power.
2. The method according to claim 1 , wherein the injection of the mixture to be separated is cyclic.
3. The method according to claim 1 , wherein the drawing-off of the enriched fraction is cyclic.
4. The method according to claim 3 , wherein the displacement of the injection points of the eluents is periodic.
5. The method according to claim 1 , wherein the eluents are injected continuously or discontinuously.
6. The method according to claim 1 , wherein at least one eluent is injected discontinuously.
7. The method according to claim 1 , wherein the composition of each eluent is constant.
8. The method according to claim 1 , wherein the composition of at least one eluent, independently of each other, is variable.
9. The method according to claim 1 , wherein at least one eluent is displaced asynchronously.
10. The method according to claim 1 , wherein at least two columns separate the first single point from the second single point, according to the direction of flow of the mobile phase.
11. The method according to claim 1 , wherein all the columns of the system separate the first single point from the second single point, according to the direction of flow of the mobile phase.
12. The method according to claim 1 , wherein the mixture to be separated is injected by means of units selected from the group comprising an injection loop, and injection pump, a pressurized container.
13. The method according to claim 1 , wherein the mixture to be separated is circulated in an additional chromatography column prior to injection into the device.
14. The method according to claim 1 , wherein the additional chromatography column is inserted between the mixture injection unit and the unique point for injecting the mixture into the device or is between two columns of the device.
15. A method for separating fractions of a mixture to be separated comprising the steps of:
providing a device comprising at least two chromatography columns mounted in series,
discontinuously injecting a mixture to be separated, in a first single point of the device,
discontinuously drawing off the fraction enriched in the product of interest, in a second single point of the device, and
injecting eluents in a plurality of points of each column and displacing the plurality of points for injecting eluents, the eluents having a different eluting power; wherein the mixture is a mixture of synthetic molecules, natural extracts, proteins obtained by fermentation, proteins obtained by synthesis or hemi-synthesis, immunoglobulins, monoclonal antibodies, peptides obtained by fermentation, or peptides obtained by synthesis or hemi-synthesis.
16. The method according to claim 1 , wherein the method is a non-batch method.