Bioanalytical instrumentation using a light source subsystem
The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups.
1. A system comprising:
a bioanalytical tool, wherein the bioanalytical tool is a bioanalytical tool selected from the group consisting of: microarray scanners; microtiter plate readers; DNA sequencers; PCR instruments; Q-PCR instruments; microscopes; flow cytometry instruments; lab-on-a-chip devices; diagnostic medical devices; and therapeutic medical devices;
a lamp which includes,
an excitation source which provides optical excitation energy,
a light pipe for use in generating luminescence, wherein the light pipe includes a luminescent material which absorbs the optical excitation energy from the excitation source and, in response generates and emits luminescence at a plurality of wavelengths, and
an optical filter associated with the light pipe, wherein the optical filter selects which selected wavelengths of the plurality of wavelengths of luminescence produced by the light pipe are provided to the bioanalytical tool; and
a fiber optic device connecting the lamp to the bioanalytical tool, wherein the fiber optic device transmits the selected wavelengths of luminescence from the lamp to the bioanalytical tool.
2. The system of claim 1 , wherein the optical excitation energy has a shorter wavelength than the luminescence.
3. The system of claim 2 , wherein the luminescence comprises green light.
4. The system of claim 3 , wherein the optical excitation energy comprises UV light.
5. The system of claim 1 , wherein the excitation source comprises an LED light source.
6. The system of claim 1 , wherein the luminescent material comprises a rare earth doped crystalline material.
7. The system of claim 1 , further comprising a driver circuit which modulates the excitation source at a modulation rate of at least one MHz in order to modulate the luminescence emitted by the light pipe.
8. The system of claim 1 , wherein:
the excitation source comprises a plurality of LEDs; and
the system further comprises a square wave generator connected to a transistor amplifier circuit which drives the plurality of LEDs thereby modulating the luminescence emitted by the light pipe at rates exceeding one megahertz.
9. The system of claim 1 , wherein:
the excitation source comprises a plurality of LEDs; and
wherein feedback of the selected wavelengths of light produced by the light pipe is used to drive a voltage applied to the plurality of LEDs to provide selected wavelengths of light produced by the light pipe at constant power.
10. The system of claim 1 , wherein the luminescent material comprises a rod comprising a rare earth element doped yttrium aluminum garnet (YAG) crystalline material.
11. A system comprising:
a bioanalytical tool, wherein the bioanalytical tool is a bioanalytical tool selected from the group consisting of: microarray scanners; microtiter plate readers; DNA sequencers; PCR instruments; Q-PCR instruments; microscopes; flow cytometry instruments; lab-on-a-chip devices; diagnostic medical devices; and therapeutic medical devices;
a lamp which includes,
a plurality of LEDs which provides optical excitation energy,
a square wave generator connected to a transistor amplifier circuit which drives the plurality of LEDs thereby modulating optical excitation energy emitted by the plurality of LEDs at rates exceeding one megahertz
a light pipe for use in generating luminescence, wherein the light pipe includes a luminescent material which absorbs the optical excitation energy from the excitation source and, in response generates and emits luminescence at a plurality of wavelengths, and
an optical filter associated with the light pipe, wherein the optical filter selects which selected wavelengths of the plurality of wavelengths of luminescence produced by the light pipe are provided to the bioanalytical tool; and
a fiber optic device connecting the lamp to the bioanalytical tool, wherein the fiber optic device transmits the selected wavelengths of luminescence from the lamp to the bioanalytical tool.
12. The system of claim 11 , wherein the optical excitation energy has a shorter wavelength than the luminescence.
13. The system of claim 11 , wherein the luminescence comprises green light.
14. The system of claim 11 , wherein the optical excitation energy comprises UV light.
15. The system of claim 11 , wherein the luminescent material comprises a rare earth doped element crystalline material.
16. The system of claim 11 , wherein the light pipe comprises a rod comprising a rare earth element doped yttrium aluminum gamet (YAG) crystalline luminescent material.
17. A system comprising:
a bioanalytical tool, wherein the bioanalytical tool is a bioanalytical tool selected from the group consisting of: microarray scanners; microtiter plate readers; DNA sequencers; PCR instruments: Q-PCR instruments; microscopes; flow cytometry instruments; lab-on-a-chip devices; diagnostic medical devices; and therapeutic medical devices;
a lamp which includes,
a plurality of LEDs which provides optical excitation energy,
a square wave generator connected to a transistor amplifier circuit which drives the plurality of LEDs thereby modulating optical excitation energy emitted by the plurality of LEDs at rates exceeding one megahertz
a rod comprising a rare earth element doped yttrium aluminum garnet (YAG) crystalline luminescent material which absorbs the optical excitation energy from the excitation source and, in response generates and emits luminescence at a plurality of wavelengths, and
an optical filter associated with the light pipe, wherein the optical filter selects which selected wavelengths of the plurality of wavelengths of luminescence produced by the rod are provided to the bioanalytical tool; and
a fiber optic device connecting the lamp to the bioanalytical tool, wherein the fiber optic device transmits the selected wavelengths of luminescence from the lamp to the bioanalytical tool.
18. The system of claim 17 , wherein the optical excitation energy has a shorter wavelength than the luminescence.
19. The system of claim 17 , wherein the luminescence comprises green light.
20. The system of claim 17 , wherein the optical excitation energy comprises UV light.