Neutralizing human anti-IGFR antibody
View Patent ↗The present invention includes fully human, neutralizing, monoclonal antibodies against human Insulin-like Growth Factor Receptor-I (IGFR1). The antibodies are useful for treating or preventing cancer in a subject. Also included are methods of using and producing the antibodies of the invention.
1. An isolated antibody or antigen binding fragment thereof produced by a method comprising introducing a polynucleotide encoding a polypeptide that comprises amino acids 20-137 of SEQ ID NO: 45 and a polynucleotide encoding a polypeptide that comprises amino acids 20-128 of SEQ ID NO: 78 into a host cell under conditions wherein said antibody or fragment are produced in said host cell.
2. The antibody or fragment of claim 1 wherein the host cell is a eukaryotic cell.
3. The antibody or fragment of claim 2 wherein said host cell is Chinese hamster ovary cell.
4. The antibody or fragment of claim 2 wherein the host cell is a fungal cell.
5. The antibody or fragment of claim 1 wherein said polypeptides are isolated from said cell.
6. The antibody or fragment of claim 1 wherein a polynucleotide encoding a polypeptide is operably associated with a CMV promoter.
7. The antibody or fragment of claim 1 wherein the polynucleotides are in a vector.
8. The antibody or fragment of claim 7 wherein the vector comprises a DHFR gene.
9. The antibody or frayment of claim 8 wherein the DHFR gene is operably linked to a mouse mammary tumor virus long terminal repeat promoter.
10. The antibody or fragment of claim 7 wherein the vector comprises a hygromycin B gene.
11. The antibody or fragment of claim 10 wherein the hygromycin B gene is operably linked to a TK promoter.
12. The antibody or fragment of claim 1 wherein the method comprises introducing one or more vectors comprising one or more polynucleotides encoding said polypeptides, operably linked to a CMV promoter, into a Chinese hamster ovary cell under conditions wherein said antibody or fragment is produced in said host cell and isolating said polypeptides.
13. The antibody or fragment of claim 12 wherein a vector comprises a DHFR gene.
14. The antibody or fragment of claim 13 wherein the DHFR gene is operably linked to a mouse mammary tumor virus long terminal repeat promoter.
15. The antibody or fragment of claim 14 wherein a vector comprises a hygromycin B gene.
16. The antibody or fragment of claim 15 wherein the hygromycin B gene is operably linked to a TK promoter.