Subpopulations of spore-like cells and uses thereof
View Patent ↗Subpopulations of spore-like cells expressing specific cell surface and gene expression markers are provided. In one embodiment, the cells express at least one cell surface or gene expression marker selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10. Also provided are methods for purifying a subpopulation of spore-like cells of interest from a population of spore-like cells, and methods for inducing differentiation of the isolated spore-like cells into cells of endodermal, mesodermal or ectodermal origin. The spore-like cells can be used to generate cells originating from all three germ layers and can be used to treat a patient who has a deficiency of functional cells in any of a wide variety of tissues, including the retina, intestine, bladder, kidney, liver, lung, nervous system, or endocrine system.
1. An isolated sub-population of spore-like cells expressing one or more markers selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10, wherein the cells remain viable following oxygen deprivation for at least 24 hours.
2. The sub-population of spore-like cells of claim 1 , wherein the cells are isolated from post-natal animal tissue selected from the group consisting of ectodermal, mesodermal and endodermal tissue.
3. The subpopulation of spore-like cells of claim 2 , wherein the post-natal animal tissue is selected from the group consisting of mammalian, avian, reptilian and amphibian tissue.
4. The subpopulation of spore-like cells of claim 3 , wherein the tissue is selected from the group consisting of heart, intestine, bladder, kidney, liver, lung, adrenal gland, skin, retina, and pancreas.
5. The subpopulation of spore-like cells of claim 1 , wherein the cells further express nestin.
6. A method of isolating a subpopulation of spore-like cells comprising the steps of:
(a) isolating spore-like cells from a tissue,
(b) culturing the cells for a time sufficient for expression of one or more markers selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10, and
(c) identifying and isolating cells expressing the one or more markers wherein the cells remain viable following oxygen deprivation for at least 24 hours.
7. The method of claim 6 wherein the cells are isolated using antibodies specific for the marker.
8. The method of claim 7 comprising the steps of:
(a) contacting the population of cells with a binding partner that is specific for a marker selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10, under conditions sufficient to allow binding of the binding partner to its target, if present, on each cell of the population of spore-like cells; and
(b) isolating the marker-positive cells to yield a subpopulation of spore-like cells.
9. The method of claim 8 wherein the cells are isolated using fluorescence-activated cell sorting.