Polypeptides having lipase activity and polynucleotides encoding same
View Patent ↗The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
1. An isolated polypeptide having lipase activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 90% identity with the mature polypeptide of SEQ ID NO: 6;
(b) a polypeptide which is encoded by a polynucleotide comprising a nucleotide sequence which hybridizes under at least high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 5 or its full-length complementary strand, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and a wash three times, each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 90% identity with the mature polypeptide coding sequence of SEQ ID NO: 5.
2. The isolated polypeptide of claim 1 , which comprises an amino acid sequence having at least 90% identity with the mature polypeptide of SEQ ID NO: 6.
3. The isolated polypeptide of claim 2 , which comprises an amino acid sequence having at least 95% identity with the mature polypeptide of SEQ ID NO: 6.
4. The isolated polypeptide of claim 3 , which comprises an amino acid sequence having at least 97% identity with the mature polypeptide of SEQ ID NO: 6.
5. The isolated polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence which hybridizes under at least high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 5 or its full-length complementary strand, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and a wash three times, each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.
6. The isolated polypeptide of claim 5 , which is encoded by a polynucleotide comprising a nucleotide sequence which hybridizes under at least very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 5 or its full-length complementary strand, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and a wash three times, each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.
7. The polypeptide of claim 1 , which comprises or consists of the amino acid sequence of SEQ ID NO: 6.
8. The polypeptide of claim 7 , which comprises or consists of the amino acid sequence of SEQ ID NO: 6.
9. The polypeptide of claim 7 , which comprises or consists of the mature polypeptide of SEQ ID NO: 6.
10. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 90% identity with the mature polypeptide coding sequence of SEQ ID NO: 5.
11. The polypeptide of claim 10 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity with the mature polypeptide coding sequence of SEQ ID NO: 5.
12. The polypeptide of claim 11 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% identity with the mature polypeptide coding sequence of SEQ ID NO: 5.
13. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 5.
14. The polypeptide of claim 13 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 5.
15. The polypeptide of claim 13 , which is encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO: 5.
16. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pJLin175 which is contained in E. coli NRRL B-30783.
17. The polypeptide of claim 1 , wherein the mature polypeptide is amino acids 21 to 598 of SEQ ID NO: 6.
18. The polypeptide of claim 1 , wherein the mature polypeptide coding sequence is nucleotides 61 to 1922 of SEQ ID NO: 5.
19. A detergent composition comprising the polypeptide of claim 1 and a surfactant.