Stabilization of vitamin B
View Patent ↗The invention relates to preparations comprising vitamin B 12 and a butanol, and to the use of butanol for stabilizing vitamins.
1. A method of stabilizing a vitamin B12 formulation to light, the method comprising:
dissolving an amount of vitamin B12 in water by heating to form a vitamin B12 solution,
mixing the vitamin B12 solution with butaphosphan and n-butanol to form a stock solution, and
adjusting the stock solution to a pH of from about 4 to about 7 by adding an acid or a base to form the vitamin B12 formulation, wherein the vitamin B12 formulation comprises from 0.001 to 0.01% m/V vitamin B12 and from 0.5 to 7% m/V n-butanol.
2. The method of claim 1 , wherein the vitamin B12 formulation comprises from 1 to 30% m/V butaphosphan.
3. The method of claim 1 , wherein the vitamin B12 formulation comprises from 0.001 to 10% m/V acid or base.
4. The method of claim 1 , wherein the vitamin B12 formulation further comprises a cosolvent.
5. The method of claim 1 , wherein the vitamin B12 formulation further comprises an antioxidant, wetting agent, or combinations thereof.
6. The method of claim 1 wherein the pH of the stock solution is adjusted to 5.6±0.2 by adding an acid or a base.
7. The method of claim 1 wherein the vitamin B12 formulation comprises from 0.005 to 0.01% m/V vitamin B12.
8. The method of claim 1 wherein the vitamin B12 formulation comprises from 0.0075 to 0.01% m/V vitamin B12.
9. The method of claim 1 wherein the vitamin B12 formulation comprises from 1 to 5% m/V n-butanol.
10. The method of claim 1 wherein the vitamin B12 formulation comprises from 2 to 4% m/V n-butanol.
11. The method of claim 1 further comprising sterilizing the vitamin B12 formulation.
12. The method of claim 1 wherein vitamin B12 is cyanocobalamin.