Method for producing butanol using extractive fermentation with electrolyte addition
View Patent ↗A method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, is provided. The electrolyte may comprise a salt which dissociates in the fermentation medium, or in the aqueous phase of a biphasic fermentation medium, to form free ions. Also provided is a method and composition for recovering butanol from a fermentation medium.
1. A method for recovering butanol from a fermentation medium, the method comprising:
a) providing a fermentation medium comprising butanol, water, at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, and a genetically modified microorganism that produces butanol from least one fermentable carbon source;
b) contacting the fermentation medium with i) a first water-immiscible organic extractant selected from the group consisting of C 12 to C 22 fatty alcohols, C 12 to C 22 fatty acids, esters of C 12 to C 22 fatty acids, C 12 to C 22 fatty aldehydes, C 12 to C 22 fatty amides, and mixtures thereof, and optionally ii) a second water-immiscible organic extractant selected from the group consisting of C 7 to C 22 fatty alcohols, C 7 to C 22 fatty acids, esters of C 7 to C 22 fatty acids, C 7 to C 22 fatty aldehydes, C 7 to C 22 fatty amides, and mixtures thereof to form a two-phase mixture comprising an aqueous phase and a butanol-containing organic phase; and
c) recovering the butanol from the butanol-containing organic phase to produce recovered butanol.
2. The method of claim 1 , wherein a portion of the butanol is concurrently removed from the fermentation medium by a process comprising the steps of:
a) stripping butanol from the fermentation medium with a gas to form a butanol-containing gas phase; and
b) recovering butanol from the butanol-containing gas phase.
3. The method of claim 1 , wherein the electrolyte is added to the fermentation medium, to the first extractant, to the optional second extractant, or to combinations thereof.
4. The method of claim 1 , wherein the electrolyte comprises a salt having a cation selected from the group consisting of lithium, sodium, potassium, rubidium, cesium, magnesium, calcium, strontium, barium, ammonium, phosphonium, and combinations thereof.
5. The method of claim 1 , wherein the electrolyte comprises a salt having an anion selected from the group consisting of sulfate, carbonate, acetate, citrate, lactate, phosphate, fluoride, chloride, bromide, iodide, and combinations thereof.
6. The method of claim 1 , wherein the electrolyte is selected from the group consisting of sodium sulfate, sodium chloride, and combinations thereof.
7. The method of claim 1 , wherein the genetically modified microorganism is selected from the group consisting of bacteria, cyanobacteria, filamentous fungi, and yeasts.
8. The method of claim 7 wherein the bacteria are selected from the group consisting of Zymomonas, Escherichia, Salmonella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Pediococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium , and Brevibacterium.
9. The method of claim 7 wherein the yeast is selected from the group consisting of Pichia, Candida, Hansenula, Kluyveromyces, Issatchenkia , and Saccharomyces.
10. The method of claim 1 , wherein the first extractant is selected from the group consisting of oleyl alcohol, behenyl alcohol, cetyl alcohol, lauryl alcohol, myristyl alcohol, stearyl alcohol, oleic acid, lauric acid, myristic acid, stearic acid, methyl myristate, methyl oleate, lauric aldehyde, 1-dodecanol, and a combination of these.
11. The method of claim 1 , wherein the first extractant comprises oleyl alcohol.
12. The method of claim 1 , wherein the second extractant is selected from the group consisting of 1-nonanol, 1-decanol, 1-undecanol, 2-undecanol, 1-nonanal, and a combination of these.
13. The method of claim 1 , wherein the butanol is 1-butanol.
14. The method of claim 1 , wherein the butanol is 2-butanol.
15. The method of claim 1 , wherein the butanol is isobutanol.
16. The method of claim 1 , wherein the fermentation medium further comprises ethanol, and the butanol-containing organic phase contains ethanol.
17. The method of claim 1 wherein the genetically modified microorganism comprises a modification which inactivates a competing pathway for carbon flow.
18. The method of claim 1 wherein the genetically modified microorganism does not produce acetone.
19. The method of claim 1 , wherein said at least one fermentable carbon source is present in the fermentation medium and comprises renewable carbon from agricultural feedstocks, algae, cellulose, hemicellulose, lignocellulose, or any combination thereof.