Tissue adhesives and sealants and methods for their use
View Patent ↗Compositions provided by mixing a biotin-containing component and an avidin-containing component are useful as an adhesive or sealant for medical/surgical uses.
1. A method comprising:
maintaining a biotin-containing component comprising a polymer selected from the group consisting of polyethylene glycols and absorbable polymers separately from an avidin-containing component comprising a polymer selected from the group consisting of polyethylene glycols and absorbable polymers;
simultaneously applying the biotin-containing component and avidin-containing component directly to tissue; and
allowing biotin groups on the biotin-containing component to react with avidin groups on the avidin-containing component to form a three-dimensional crosslinked matrix as a tissue adhesive or sealant,
wherein the absorbable polymer of the biotin-containing component or the avidin-containing component is selected from the group consisting of polycaprolactone, poly-D,L-lactic acid, poly-L-lactic acid, poly(lactide-co-glycolide), poly(hydroxybutyrate), poly(hydroxybutyrate-co-valerate), polydioxanone, polyorthoester, polyanhydride, poly(glycolic acid), poly(glycolic acid-cotrimethylene carbonate), polyphosphoester, polyphosphoester urethane, polyglutamic acid, polyaspartic acid, absorbable cyanoacrylates, poly(trimethylene carbonate), copoly(ether-esters), polyalkylene oxalates, polyphosphazenes, polyiminocarbonates, aliphatic polycarbonates, and combinations thereof.
2. The method according to claim 1 , wherein the biotin-containing component comprises a branched polymer functionalized with biotin.
3. The method according to claim 1 , wherein the biotin-containing component comprises polyethylene glycol having a molecular weight of less than 1000.
4. The method according to claim 1 , wherein the avidin-containing component comprises a polymer functionalized with avidin.
5. The method according to claim 1 , wherein the avidin-containing component comprises a polymer functionalized with streptavidin.
6. The method according to claim 1 , wherein the avidin-containing component comprises a branched polymer.
7. The method according to claim 1 , wherein the avidin-containing component comprises a polyethylene glycol having a molecular weight of less than 1000.
8. The method according to claim 1 , wherein the three-dimensional crosslinked matrix further comprises a bioactive agent.