Protein electron mediator
View Patent ↗The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor. The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.
1. A sensor for performing measurement of a subject to be measured included in a sample solution, comprising at least:
i) an insulating first substrate;
ii) first and second electrodes placed on said first substrate;
iii) a reagent layer placed on said first electrode; and
iv) a sample solution-holding part contacting said first electrode or said reagent layer and said second electrode, wherein said reagent layer includes extracellular secretion type cytochrome and an enzyme is placed on either said reagent layer or said sample solution-holding part;
wherein said enzyme is flavin adenine dinucleotide-dependent.
2. The sensor according to claim 1 , wherein either of said first and second electrodes includes any of carbon, gold, platinum or palladium.
3. The sensor according to claim 1 , wherein said second electrode is an oxidizing and reducing electrode.
4. The sensor according to claim 3 , wherein said second electrode is Ag/AgCl.
5. The sensor according to claim 1 , wherein said reagent layer is placed only on said first electrode.
6. The sensor according to claim 1 , wherein said extracellular secretion type cytochrome and said enzyme are fused.
7. The sensor according to claim 1 , wherein the amount of said extracellular secretion type cytochrome is smaller than 100 times that of said enzyme used in measurement.
8. The sensor according to claim 1 , wherein said extracellular secretion type cytochrome is derived from any of a bacterium belonging to filamentous bacteria, or a bacterium belonging to genus Aspergillus.
9. The sensor according to claim 1 , wherein said enzyme is oxidoreductase or dehydrogenase.
10. The sensor according to claim 1 , wherein said enzyme is an enzyme acting on glucose.
11. The sensor according to claim 1 , wherein said enzyme is flavin adenine dinucleotide-dependent glucose dehydrogenase.
12. The sensor according to claim 1 , wherein said subject to be measured is glucose.
13. The sensor according to claim 1 that further includes a second substrate having a v) notch part, wherein said second substrate forms at least a portion of said sample solution-holding part.
14. The sensor according to claim 13 , wherein the shape of said notch part is rectangular or U-shaped.
15. The sensor according to claim 13 that further includes a vi) third substrate, wherein said third substrate forms at least a portion of said sample solution-holding part.
16. The sensor according to claim 1 , wherein a measurable concentration of said subject to be measured is greater than 5 mM.