Patent
Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.
1. A Brassica plant having in its genome in the following order: a polynucleotide comprising SEQ ID NO: 12, a polynucleotide encoding a glyphosate-N-acetyltransferase and a polynucleotide comprising SEQ ID NO: 13.
2. A Brassica plant that is a progeny of a plant grown from the seeds of ATCC Seed Deposit PTA-11504, wherein said progeny comprises a DP-073496-4 event.
3. A plant material derived from said Brassica plant of claim 2 , wherein said plant material comprises a DP-073496-4 event.
4. The plant material of claim 3 , wherein said plant material comprises a food product.
5. A transgenic Brassica seed having in its genome in the following order: a polynucleotide comprising SEQ ID NO: 12, a polynucleotide encoding a glyphosate-N-acetyltransferase and a polynucleotide comprising SEQ ID NO: 13.
6. A transgenic seed of ATCC Seed Deposit PTA-11504.
7. A plant material derived from the transgenic seed of claim 6 , wherein said plant material comprises a DP-073496-4 event.
8. The plant material of claim 7 , wherein said plant material comprises a food product.
9. A method of producing a glyphosate tolerant plant comprising breeding a Brassica plant having in its genome in the following order: a polynucleotide comprising SEQ ID NO: 12, a polynucleotide encoding a glyphosate-N-acetyltransferase and a polynucleotide comprising SEQ ID NO: 13 or a plant that is a progeny of a plant grown from the seeds of ATCC Seed Deposit PTA-11504, wherein said progeny comprises a DP-073496-4 event,
and selecting progeny by analyzing for at least one polynucleotide comprising a DP-073496-4 specific region.
10. The method of claim 9 , wherein said polynucleotide comprising the DP-073496-4 specific region comprises SEQ ID NO:12 or 13.
11. The method of claim 10 , wherein said polynucleotide comprising the DP-073496-4 specific region comprises:
(a) a nucleotide sequence set forth in SEQ ID NO: 14, 15, 16, or 17; or,
(b) a nucleotide sequence comprising a fragment of SEQ ID NO: 14, 15, 16, or 17.
12. A method for controlling weeds in an area of cultivation comprising applying an effective amount of glyphosate to the area of cultivation having a Brassica plant or seed having in its genome in the following order: a polynucleotide comprising SEQ ID NO: 12, a polynucleotide encoding a glyphosate-N-acetyltransferase and a polynucleotide comprising SEQ ID NO: 13; or a Brassica plant or seed that is a progeny of a plant grown from the seeds of ATCC Seed Deposit PTA-11504, wherein said progeny comprises a DP-073496-4 event.
13. A method of growing a glyphosate tolerant Brassica plant comprising
(a) planting in an area of cultivation a Brassica plant or seed having in its genome a polynucleotide comprising SEQ ID NO: 12, a polynucleotide encoding a glyphosate-N-acetyltransferase and a polynucleotide comprising SEQ ID NO: 13, or a Brassica plant or seed that is a progeny of a plant grown from the seeds of ATCC Seed Deposit PTA-11504, wherein said progeny comprises a DP-073496-4 event, and
(b) applying glyphosate to the area of cultivation.