Nanofibrous scaffold comprising immobilized cells
The invention is directed to a device and method to prevent migration of Human Mesenchymal Stem Cells (hMSCs) from a delivery site while allowing communication between the stem cells and native cardiomyocytes. The device is characterized by scaffold pore size, fiber diameter and biomaterial selection. The invention includes a two part polyurethane scaffold that prevents migration of stem cells, allows gap junction formation through pores and is packaged for minimally invasive delivery.
1. A device comprising a nanofibrous scaffold is 10-150 μm thick and contains pores that are 0.5-3 μm in diameter and immobilized cells.
2. The device of claim 1 , wherein said nanofibrous scaffold is non-degradable.
3. The device of claim 1 , wherein said scaffold comprises a synthetic material selected from the group consisting of nitinol, dacron, nylon, polytetrafluoroethyline, poly(glycolic acid), and polyurethane.
4. The device of claim 1 , wherein said scaffold comprises electrospun polyurethane.
5. The device of claim 1 , wherein said scaffold is in the form of a chamber or a layered sandwich.
6. The device of claim 5 , wherein said chamber contains stem cells.
7. The device of claim 6 , wherein said stem cells are human mesenchymal stem cells.
8. The device of claim 1 , wherein said pores prohibit the migration of stem cells.
9. The device of claim 1 , wherein said pores allow gap junction formation and protein diffusion.
10. The device of claim 1 , wherein said pores are less than 3 μm in diameter.
11. The device of claim 1 , wherein said pores are 2-2.5 μm in diameter.
12. The device of claim 1 , wherein said nanofibrous scaffold is 10-20 μm thick.
13. The device of claim 1 , wherein said nanofibrous scaffold is 30-60 μm thick.
14. The device of claim 1 , wherein said nanofibrous scaffold is greater than 90 μm thick.
15. The device of claim 5 , wherein said chamber shape is selected from the group comprising disc-shaped, cylindrical, oval, football-shaped and round.