Polymorphisms associated with Parkinson's disease
The invention provides human polymorphisms that are associated with Parkinson's disease (PD). Also disclosed are compositions and methods for use in diagnostics, prognostics, prevention, treatment and/or study of PD.
1. A method for screening a human subject for susceptibility to Parkinson's Disease (PD), the method comprising:
obtaining a nucleic acid sample from the human subject;
determining which allele is present in the sample at the polymorphic nucleotide position of SNP rs10513789 (SEQ ID NO: 1); and
identifying the human subject as having an increased risk of developing PD if the subject has a T at the polymorphic nucleotide position of rs10513789 (SEQ ID NO: 1).
2. The method of claim 1 wherein determining the identity of the polymorphic allele(s) is by a process that includes one or more of: sequencing the polymorphic allele(s) in a genomic DNA isolated from the nucleic acid sample, hybridizing the polymorphic allele(s) or an amplicon thereof to an array, digesting the polymorphic allele(s) or an amplicon thereof with a restriction enzyme, or amplification of the polymorphic allele(s).
3. The method of claim 2 , wherein the amplification comprises performing a polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), or ligase chain reaction (LCR) using a nucleic acid isolated from the biological sample as a template in the PCR, RT-PCR, or LCR.
4. The method of claim 1 wherein the sample is obtained from blood or saliva.
5. The method of claim 1 , further comprising determining which allele is present in the sample at one or more of the polymorphic nucleotide positions selected from the group of SNPs consisting of rs6599389 (SEQ ID NO: 2), rs873785 (SEQ ID NO: 3), rs11248060 (SEQ ID NO: 4), rs6812193 (SEQ ID NO: 5), rs4130047 (SEQ ID NO: 6), rs7451962 (SEQ ID NO: 7) and rs4397141 (SEQ ID NO: 8).
6. A method for generating a prognosis of a human subject's susceptibility to Parkinson's Disease (PD), comprising:
obtaining a genomic sample from said human subject;
analyzing the genomic sample to determine which allele is present in the sample at the polymorphic nucleotide position of SNP rs10513789 (SEQ ID NO: 1);
storing the determined allele of the sample in a database that includes a set of information related to said subject;
correlating the determined allele with an association between the alleles of rs10513789 (SEQ ID NO: 1) and susceptibility to PD in the database;
generating a prognosis of the subject's susceptibility to PD based on the correlation; and
communicating the prognosis of susceptibility to a medical practitioner.
7. The system of claim 6 wherein the set of information related to said subject comprises family medical history, diet, exercise and medical history of said subject.