Solid-phase extraction (SPE) tips and methods of use

1. A method comprising:

introducing a solvent into an extraction tip;

prior to extracting analytes from a dried blood spot card, causing the solvent to contact a solid phase extraction (SPE) or chromatographic packing material within the extraction tip;

extracting the analytes from the dried blood spot card by:

dispensing the solvent from a first end of the extraction tip to form a micro liquid junction that extends between the first end of the extraction tip and a surface of the dried blood spot card outside of the extraction tip;

allowing the solvent to dissolve the analytes from the dried blood spot card; and

aspirating the solvent and dissolved analytes into the extraction tip from the surface of the dried blood spot card through the first end of the extraction tip such that the analytes are retained in the SPE packing material in the extraction tip by applying a vacuum at a second end of the extraction tip;

interfacing the extraction tip to an automation device to deliver the extraction tip used to extract the analytes to an ion sprayer; and

directing ions from the ion sprayer to a mass analyzer.

2. The method of claim 1 , further comprising: repeating the steps of dispensing, applying and aspirating at different locations of the dried blood spot card.

3. The method of claim 1 , further comprising: repeating the steps of dispensing, applying and aspirating at the same location on the dried blood spot card.

4. The method of claim 1 , wherein the ion sprayer is an electrospray ionization source having multiple openings through which the extraction tip can singularly or in multiple deliver the analyte.

5. The method of claim 1 , further comprising aligning the extraction tip to be in close proximity to the dried blood spot card.

6. The method of claim 1 , wherein the first end of the extraction tip has a smaller diameter than the second end.

7. The method of claim 6 , wherein a diameter of the first end is between 400 μm to 1200 μm and a diameter of the second end is between 1 to 10 mm.

8. The method of claim 1 , wherein dispensing the solvent comprises dispensing microliters of solvent from the first end of the extraction tip to contact the dried blood spot card.

9. The method of claim 1 , wherein the SPE packing material includes a material suitable for Hydrophilic Interaction Liquid Chromatography (HILIC).

10. The method of claim 9 , wherein the solvent is a normal phase eluting solvent.

11. The method of claim 10 , wherein the normal phase eluting solvent includes methanol or acetonitrile.

12. The method of claim 1 , wherein the solvent is introduced into the extraction tip via the first end.

13. The method of claim 1 , wherein aspirating the solvent and dissolved analytes into the extraction tip via the first end of the extraction tip comprises extracting multiple analytes from the dried blood spot card.

14. A method of extracting analytes from a sample, the method comprising:

cutting a dried blood spot card to generate portion of the card including the sample to fit within an inner diameter an extraction tip;

positioning the portion of the card including the sample within the extraction tip;

introducing a solvent to a first end of the extraction tip;

causing analytes within the sample to dissolve in the solvent;

causing the solvent to contact a solid phase extraction (SPE) packing material within the extraction tip;

eluting the analytes retained by the SPE packing material; and

causing the dissolved analytes to exit the extraction tip from a second end of the extraction tip.

15. The method of claim 14 , wherein the first end has a larger diameter than the second end.