Methods for assessing RNA patterns
View Patent ↗Methods and compositions for the characterizing of cancers by assessing RNA levels, such as determining an RNA pattern, are provided herein. The diagnosis, prognosis, monitoring and treatment or a cancer can be determined by detecting one or more RNAs, such as microRNAs.
1. A method of characterizing prostate cancer in a human subject comprising:
a. determining the level of one or more proteins in a biological sample from the subject, wherein the one or more proteins comprise a protein selected from the group consisting of PSMA, EpCam, CD9, CD63, CD81, Rab-5 and a combination thereof;
b. determining the level of miR-141 in the sample; and
c. comparing the levels of the one or more proteins in (a) and miR-141 in (b) to a reference, wherein a difference in the levels as compared to the reference is used to characterize the prostate cancer.
2. The method of claim 1 , wherein the characterizing comprises a characterization selected from the group consisting of diagnosis, prognosis, staging, grading, determination of drug efficacy, monitoring the status of the subject's response or resistance to a treatment, selection of a treatment for the prostate cancer, and a combination thereof.
3. The method of claim 2 , wherein the subject is non-responsive to a current therapeutic being administered to the subject.
4. The method of claim 3 , wherein the therapeutic is a cancer therapeutic.
5. The method of claim 1 , wherein the reference is derived from a subject without prostate cancer.
6. The method of claim 1 , wherein the reference is derived from the subject over a time course.
7. The method of claim 1 , wherein the biological sample comprises a bodily fluid.
8. The method of claim 1 , wherein the bodily fluid comprises a bodily fluid selected from the group consisting of peripheral blood, serum, plasma, urine, semen, prostatic fluid, cowper's fluid, pre-ejaculatory fluid, and a combination thereof.
9. The method of claim 1 , wherein the bodily fluid comprises plasma or serum.
10. The method of claim 1 , wherein the one or more proteins comprise CD9.
11. The method of claim 1 , wherein the one or more proteins comprise CD63.
12. The method of claim 1 , wherein the one or more proteins comprise CD81.
13. The method of claim 1 , wherein the one or more proteins comprise PSMA.
14. The method of claim 1 , wherein the one or more proteins comprise EpCam.
15. The method of claim 1 , wherein the one or more proteins in (a) and miR-141 in (b) are associated with one or more membrane vesicles in the sample.
16. The method of claim 15 , wherein the one or more membrane vesicles comprise membrane vesicles with a diameter of about 30 nm to about 800 nm.
17. The method of claim 15 , wherein the one or more membrane vesicles comprise membrane vesicles with a diameter of about 30 nm to about 200 nm.
18. The method of claim 15 , wherein the one or more membrane vesicles are isolated from the biological sample using an isolation technique selected from the group consisting of size exclusion chromatography, density gradient centrifugation, differential centrifugation, nanomembrane ultrafiltration, immunoabsorbent capture, affinity purification, microfluidic separation, and a combination thereof.
19. The method of claim 15 , wherein the presence or level of CD9, CD63, and/or CD81 associated with the one or more membrane vesicles is determined.
20. The method of claim 15 , wherein the one or more membrane vesicles are isolated from the biological sample using one or more binding agents for a target selected from the group consisting of PSMA, CD9, CD63, CD81, EpCam, and a combination thereof.
21. The method of claim 20 , wherein the miR-141 levels are determined in the one or more isolated membrane vesicles.
22. The method of claim 15 , wherein the one or more membrane vesicles are isolated from the biological sample using one or more binding agents for a target selected from the group consisting of PSA, TMPRSS2, FASLG, TNFSF10, PSMA, NGEP, I1-7RI, CSCR4, CysLT1R, TRPM8, Kv1.3, TRPV6, TRPM8, PSGR, MISIIR, galectin-3, PCA3, TMPRSS2:ERG, fragments thereof, and a combination thereof.
23. The method of claim 22 , wherein the one or more binding agents comprises a binding agent selected from the group consisting of DNA molecule, RNA molecule, antibody, antibody fragment, aptamer, peptoid, zDNA, peptide nucleic acid (PNA), locked nucleic acids (LNA), lectin, peptide, dendrimer, chemical compound, and a combination thereof.
24. The method of claim 22 , wherein the one or more binding agents comprises a binding agent selected from the group consisting PSA, PSMA, mAB 5D4, XPSM-A9, XPSM-A10, Galectin-3, E-selectin, Galectin-1, E4 (IgG2a kappa), and a combination thereof.