Animal protein-free media for cultivation of cells
View Patent ↗The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
1. A method for expressing a Factor VIII protein, comprising the steps of:
a) providing a culture of CHO cells into which a nucleic acid comprising a sequence encoding the Factor VIII protein has been introduced;
b) culturing the cells in an animal protein-free cell culture medium under conditions in which the Factor VIII protein is expressed, wherein the animal protein-free cell culture medium comprises putrescine at a concentration ranging from about 0.5 mg/L to about 10 mg/L and soy hydrolysate in a concentration ranging from about 0.05% (w/v) to about 0.5% (w/v), wherein the level of expression of the Factor VIII protein per cell is increased.
2. The method of claim 1 , wherein the animal protein-free cell culture medium comprises putrescine in a concentration ranging from about 2 mg/L to about 8 mg/L.
3. The method of claim 1 , wherein the animal protein-free cell culture medium comprises putrescine in a concentration ranging from about 2 mg/L to about 5 mg/L.
4. The method of claim 1 , wherein the CHO cells are cultivated by chemostat cultivation.