Interfering RNA delivery system and uses thereof
View Patent ↗The invention provides a delivery system comprising a cell penetrating peptide, a polyarginine peptide, and an interfering RNA molecule. The system can be used for delivering interfering RNA molecules into a cell in vivo or in vitro. Therapeutic uses for the delivery system are also provided.
1. An interfering RNA delivery system comprising an interfering RNA molecule linked to a cell penetrating peptide (CPP)-Arg peptide, wherein the CPP-Arg peptide comprises the sequence as set forth in SEQ ID NO: 12.
2. The delivery system of claim 1 , wherein the CPP-Arg peptide further comprises an HA2 peptide.
3. The delivery system of claim 1 , wherein the interfering RNA molecule is linked to the CPP-Arg peptide via a covalent bond.
4. The delivery system of claim 1 , wherein the interfering RNA molecule is a siRNA, miRNA, or shRNA.
5. A pharmaceutical composition comprising the delivery system of claim 1 and a pharmaceutically acceptable carrier.
6. The delivery system of claim 1 , wherein the CPP is a TAT peptide or cell penetrating fragment thereof, or the protein transduction domain of Penetratin (pAntp), Transportan, MPG, MPGde1taNLS, or pHLIP, or a cell penetrating fragment thereof.
7. The delivery system of claim 1 , wherein the CPP has an amino acid sequence in the forward or reverse direction.
8. The delivery system of claim 7 , wherein the CPP comprises at least one D-amino acid.