High-purity large-scale preparation of stannsoporfin
View Patent ↗Large scale (bulk) compositions comprising high-purity stannsoporfin are disclosed, as well as methods of synthesizing such compositions.
1. A composition comprising stannsoporfin, wherein said stannsoporfin is at least about 97.0% pure and wherein any individual impurity is present in an amount of less than about 0.1%.
2. The composition of claim 1 , wherein any individual impurity present is present in an amount of less than about 0.1%.
3. The composition of claim 1 , wherein any individual impurity present is present in an amount of less than about 0.09%.
4. The composition of claim 1 , wherein any individual impurity present is present in an amount of less than about 0.08%.
5. The composition of claim 1 , wherein any individual impurity present is present in an amount of less than about 0.07%.
6. The composition of claim 1 , wherein the composition contains less than about 20 ppm of palladium.
7. The composition of claim 1 , wherein said composition further comprises a buffer.
8. The composition of claim 7 , wherein said buffer is selected from a phosphate, citrate, gluconate, lactate, tartrate, glycinate, glycylglycinate, bicarbonate, carbonate, maleate, acetate, histidine, imidazole and combinations thereof.
9. The composition of claim 1 , wherein said composition has a pH of about 7.0 to about 8.0.
10. The composition of claim 1 , wherein said composition has a pH of about 7.4.
11. A method of treating a condition selected from hyperbilirubinemia or elevated serum levels of bilirubin in a subject, the method comprising administering a composition comprising stannsoporfin, wherein said stannsoporfin is at least about 97.0% pure and wherein any individual impurity is present in an amount of less than about 0.1%.
12. The method of claim 11 , wherein said stannsoporfin is administered by injection.
13. The method of claim 11 , wherein said stannsoporfin is administered by intramuscular injection.
14. The method of claim 11 , wherein said subject is an infant.
15. The method of claim 11 , wherein a therapeutically effective amount is 0.5 mg/kg to 25 mg/kg body weight.
16. The method of claim 11 , wherein a therapeutically effective amount is about 4.5 mg/kg body weight.
17. A method of inserting a metal into a porphyrin compound or a salt thereof comprising:
contacting a metal oxide with the porphyrin compound or salt thereof.
18. The method of claim 17 , wherein the porphyrin is selected from a mesoporphyrin, a protoporphyrin, a hematoporphyrin, a deuteroporphyrin, or salt thereof.
19. The method of claim 17 , wherein the porphyrin compound is mesoporphyrin IX or a salt thereof.
20. The method of claim 17 , wherein the porphyrin compound is mesoporphyrin IX dihydrochloride.
21. The method of claim 17 , wherein said metal oxide is selected from tin oxide, zinc oxide, copper oxide, cadmium oxide, cobalt oxide, chromium oxide, iron oxide, aluminum oxide, titanium oxide, nickel oxide, manganese oxide, silver oxide, gold oxide, vanadium oxide, platinum oxide, antimony oxide, or arsenic oxide.
22. The method of claim 17 , wherein said metal oxide is selected from tin (II) oxide, zinc (II) oxide, copper (I) oxide, copper (II) oxide, cadmium (II) oxide, cobalt (II) oxide, cobalt (III) oxide, cobalt (IV) oxide, C0304, chromium (II) oxide, chromium (III) oxide, chromium (IV) oxide, chromium (V) oxide, chromium (VI) oxide, iron (II) oxide, iron (III) oxide, Fe304, aluminum (III) oxide, titanium (II) oxide, titanium (III) oxide, titanium (IV) oxide, nickel (II) oxide, manganese (II) oxide, manganese (III) oxide, manganese (IV) oxide, manganese (VII) oxide, silver (I) oxide, silver (II) oxide, gold (I) oxide, gold (III) oxide, vanadium (II) oxide, vanadium (III) oxide, vanadium (IV) oxide, vanadium (V) oxide, platinum (II) oxide, platinum (IV) oxide, antimony (III) oxide, antimony (IV) oxide, antimony (V) oxide, arsenic (III) oxide, or arsenic (V) oxide.
23. The method of claim 17 , wherein the metal is tin, and the metal oxide is tin (II) oxide.
24. A method of inserting tin into a porphyrin compound or a salt thereof comprising:
providing a porphyrin compound or salt thereof,
providing tin (II) oxide, and
contacting the tin (II) oxide with the porphyrin compound or salt thereof under acidic conditions,
whereby the tin (II) oxide inserts into the porphyrin ring to yield a tin (IV) porphyrin compound.
25. The method of claim 24 , wherein said tin (II) oxide is dissolved or suspended in acetic acid or formic acid.
26. The method of claim 24 , wherein the porphyrin compound or salt thereof is dissolved or suspended in formic acid or acetic acid.
27. The method of claim 24 , wherein the equivalent ratio of the total amount of tin (II) oxide used to the total amount of porphyrin compound or salt thereof used is about two to six.
28. A method of inserting tin into a porphyrin compound or a salt thereof comprising:
providing a solution or suspension of a porphyrin compound or salt thereof,
providing a solution or suspension of tin (II) oxide,
adding the solution or suspension of the porphyrin compound or salt thereof to the tin (II) oxide solution over a period of about three to nine hours.
29. The method of claim 28 , wherein the tin (II) oxide solution or suspension is maintained at a temperature of about 25-115° C.