Method for generating aptamers with improved off-rates
The present disclosure is directed to the field of nucleic acid chemistry, specifically to 5-position modified uridines, as well as, oligonucleotides comprising said 5-position modified uridines. The present disclosure describes methods for producing aptamers comprising said 5-position modified uridines that are capable of binding to target molecules.
1. A compound having the following structure:
2. An oligonucleotide comprising at least one modified nucleotide having the following structure:
3. The oligonucleotide of claim 2 , wherein the oligonucleotide is a mixed ribonucleic acid/deoxyribonucleic acid or a deoxyribonucleic acid.
4. The oligonucleotide of claim 3 , wherein said oligonucleotide has at least one additional modified base selected from the group consisting of 5-bromo-1-uracilyl, 5-iodo-1-uracilyl, 5-bromovinyl-1-uracilyl, 5-iodovinyl-1-uracilyl, 5-azido-1-uracilyl, 4-thio-1-uracilyl, 4-thio-1-cytosinyl, 5-bromo-1-cytosinyl, 5-iodo-1-cytosinyl, 5-bromovinyl-1-cytosinyl, 5-iodovinyl-1-cytosinyl, 5-azido-1-cytosinyl, 8-azido-9-adeninyl, 8-bromo-9-adeninyl, 8-iodo-9-adeninyl, 8-azido-9-guaninyl, 8-bromo-9-guaninyl, 8-iodo-9-guaninyl, 8-azido-9-hypoxanthinyl, 8-bromo-9-hypoxanthinyl, 8-iodo-9-hypoxanthine, 8-azido-9-xanthinyl, 8-bromo-9-xanthinyl, 8-iodo-9-xanthinyl, 5-[(4-azidophenacyl)thio]-1-cytosinyl, 5-[(4-azidophenacyl)thio]-1-uracilyl, 5-N-(benzylcarboxamido)-1-uracilyl, 5-(N-isobutylcarboxamido)]-1-uracilyl, 5-(N-tryptaminocarboxyamido)-1-uracilyl, 5-(N-[2-(8-azido-9-hypoxanthinyl)ethyl]carboxamido)-1-uracilyl, 5-(N-[1-(3-trimethylammonium)propyl]carboxamido)-1-uracilyl chloride, 5-(N-naphthylmethylcarboxamido)-1-uracilyl, 5-(N-[1-(2,3-dihydroxypropyl)]carboxamido)-1-uracilyl, 7-deaza-7-iodo-9-adeninyl, 7-deaza-7-iodo-9-guaninyl, 7-deaza-7-bromo-9-adeninyl, 7-deaza-7-bromo-9-guaninyl, 1-isocytidinyl and 9-isoguaninyl.
5. The oligonucleotide of claim 3 , wherein said carbohydrate moiety has a sugar modification selected from the group consisting of 2′-methyl, 2′-O-methyl (2′-OMe), 2′-O-allyl, 2′-fluoro(2′-F), 2′-amino(2′—NH 2 ) and 2′-azido.
6. The oligonucleotide of claim 3 , wherein said carbohydrate moiety has been replaced by an epimeric sugar moiety selected from the group consisting of 1-arabinosyl, 1-xylosyl and 1-lyxosyl and α-anomeric analogs thereof.
7. The oligonucleotide of claim 3 , wherein said oligonucleotide has at least one additional modification which is modification which is a backbone modification of one or more phosphate moieties selected from the group consisting of P(O)S (“thioate”), P(S)S (“dithioate”), P(O)(NR 2 ) (“amidate”), P(O)R, P(O)OR′, CO and CH 2 , wherein R and R′ are independently H, aryl, alkenyl, cycloalkyl, cycloalkenyl, aralkyl and C 1-20 alkyl, optionally containing an ether (—O—) linkage.
8. An aptamer comprising at least one modified nucleotide having the following structure:
9. The aptamer of claim 8 , wherein the aptamer is a mixed ribonucleic acid/deoxyribonucleic acid or a deoxyribonucleic acid.
10. The oligonucleotide of claim 9 , wherein said oligonucleotide has at least one additional modified base selected from the group consisting of 5-bromo-1-uracilyl, 5-iodo-1-uracilyl, 5-bromovinyl-1-uracilyl, 5-iodovinyl-1-uracilyl, 5-azido-1-uracilyl, 4-thio-1-uracilyl, 4-thio-1-cytosinyl, 5-bromo-1-cytosinyl, 5-iodo-1-cytosinyl, 5-bromovinyl-1-cytosinyl, 5-iodovinyl-1-cytosinyl, 5-azido-1-cytosinyl, 8-azido-9-adeninyl, 8-bromo-9-adeninyl, 8-iodo-9-adeninyl, 8-azido-9-guaninyl, 8-bromo-9-guaninyl, 8-iodo-9-guaninyl, 8-azido-9-hypoxanthinyl, 8-bromo-9-hypoxanthinyl, 8-hypoxanthinyl, 8-iodohypoxanthinyl, 8-azido-9-xanthinyl, 8-bromo-9-xanthinyl, 8-iodo-9-xanthinyl, 5-[(4-azidophenacyl)thio]-1-cytosinyl, 5-[(4-azidophenacyl)thio]-1-uracilyl, 5-N-(benzylcarboxamido)-1-uracilyl, 5-(N-isobutylcarboxamido)]-1-uracilyl, 5-(N-tryptaminocarboxyamido)-1-uracilyl, 5-(N-[2-(1H-indol-3-yl)ethyl]carboxamido)-1-uracilyl, 5-(N-[1-(3-trimethylammonium)propyl]carboxamido)-1-uracilyl chloride, 5-(N-naphthylmethylcarboxamido)-1-uracilyl, 5-(N-[1-(2,3-dihydroxypropyl)]carboxamido)-1-uracilyl, 7-deaza-7-iodo-9-adeninyl, 7-deaza-7-iodo-9-guaninyl, 7-deaza-7-bromo-9-adeninyl, 7-deaza-7-bromo-9-guaninyl, 1-isocytidinyl and 9-isoguaninyl.
11. The oligonucleotide of claim 9 , wherein said carbohydrate moiety has a sugar modification selected from the group consisting of 2′-methyl, 2′-O-methyl (2′-OMe), 2′-O-allyl, 2′-fluoro (2′-F), 2′-amino (2′—NH 2 ) and 2′-azido.
12. The oligonucleotide of claim 9 , wherein said carbohydrate moiety has been replaced by an epimeric sugar moiety selected from the group consisting of 1-arabinosyl, 1-xylosyl and 1-lyxosyl and α-anomeric analogs thereof.
13. The oligonucleotide of claim 9 , wherein said oligonucleotide has at least one additional modification which is a backbone modification of one or more phosphate moieties selected from the group consisting of P(O)S (“thioate”), P(S)S (“dithioate”), P(O)(NR 2 ) (“amidate”), P(O)R, P(O)OR′, CO and CH 2 , wherein R and R′ are independently H, aryl, alkenyl, cycloalkyl, cycloalkenyl, aralkyl and C 1-20 alkyl, optionally containing an ether (—O—) linkage.