Oligonucleotides for amplifying
View Patent ↗Oligonucleotides for use in determining the presence of Chlamydophila pneumoniae in a test sample. The oligonucleotides are incorporated into detection probes, capture probes, and amplification oligonucleotides, and can be used in various combinations thereof.
1. A kit for amplifying a target sequence present in a target nucleic acid derived from Chlamydophila pneumonia , the kit comprising in packaged combination:
a first amplification oligonucleotide, the base sequence of which consists of (i) a target binding region that is fully complementary to a base sequence selected from the group consisting of SEQ ID NO: 48, SEQ ID NO: 56, and SEQ ID NO: 60, and, optionally, (ii) a 5′ promoter sequence; and
a second amplification oligonucleotide, the base sequence of which consists of (i) a target binding region that is fully complementary to the base sequence of SEQ ID NO: 83, and, optionally, (ii) a 5′ promoter sequence.
2. The kit of claim 1 , wherein the first amplification oligonucleotide consists of the target binding region and the 5′ promoter sequence.