Maize event DP-004114-3 and methods for detection thereof

1. A DNA construct comprising: a first, second, third and fourth expression cassette, wherein said first expression cassette in operable linkage comprises:

(a) a maize ubiquitin promoter;

(b) a 5′ untranslated exon of a maize ubiquitin gene;

(c) a maize ubiquitin first intron;

(d) a Cry1F encoding DNA molecule; and

(e) a poly(A) addition signal from ORF 25 terminator;

said second expression cassette in operable linkage comprises:

(1) a maize ubiquitin promoter;

(2) a 5′ untranslated exon of a maize ubiquitin gene;

(3) a maize ubiquitin first intron;

(4) a Cry34Ab1 encoding DNA molecule; and

(5) a PinII transcriptional terminator;

said third expression cassette in operable linkage comprises;

(i) a wheat peroxidase promoter;

(ii) a Cry35Ab1 encoding DNA molecule; and

(iii) a PinII transcriptional terminator; and

said fourth expression cassette in operable linkage comprises;

(a) a CaMV 35S promoter;

(b) a pat encoding DNA molecule; and

(c) a 3′ transcriptional terminator from CaMV 35S;

wherein the four cassettes are flanked by SEQ ID NO: 27 at the 5′ end and SEQ ID NO: 28 at the 3′ end.

2. A plant comprising the DNA construct of claim 1 .

3. A plant of claim 2 , wherein said plant is a corn plant.

4. A plant comprising the sequence set forth in SEQ ID NO: 6.

5. A corn plant comprising the genotype of the corn event DP-004114-3 deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11506, wherein said genotype comprises the DNA construct of claim 1 .

6. A corn plant comprising the genotype of the corn event DP-004114-3, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.

7. A plant parts of the plant comprising the corn event of claim 6 .

8. A seed comprising corn event DP-004114-3, wherein said seed comprises the DNA construct of claim 1 , wherein a representative sample of corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.

9. A corn plant, or part thereof, grown from the seed of claim 8 .

10. A transgenic seed produced from the corn plant of claim 9 , wherein the seed comprises corn event DP-004114-3.

11. A transgenic corn plant, or part thereof, grown from the seed of claim 10 .

12. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 6; SEQ ID NO: 27; SEQ ID NO: 28, and full length complements thereof.

13. An amplicon comprising the nucleic acid sequence selected from the group consisting of SEQ ID NO: 27, SEQ ID NO: 28 and full length complements thereof.

14. A biological sample derived from corn event DP-004114-3 plant, tissue, or seed, wherein said sample comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 27 and SEQ ID NO: 28, or the complement thereof, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.

15. The biological sample of claim 14 , wherein said biological sample comprise plant, tissue, or seed of transgenic corn event DP-004114-3.

16. The biological sample of claim 15 , wherein said biological sample is a DNA sample extracted from the transgenic corn plant event DP-004114-3, and wherein said DNA sample comprises one or more of the nucleotide sequences selected from the group consisting of SEQ ID NO: 27, SEQ ID NO: 28, and the complement thereof.

17. The biological sample of claim 16 , wherein said biological sample is selected from the group consisting of corn flour, corn meal, and cereals manufactured in whole or in part to contain corn by-products.

18. An extract derived from corn event DP-004114-3 plant, tissue, or seed and comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 27 and SEQ ID NO: 28 or the complement thereof, wherein a representative sample of said corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.

19. The extract of claim 18 , wherein said nucleotide sequence is detectable in said extract using a nucleic acid amplification or nucleic acid hybridization method.

20. The extract of claim 19 , further comprising a composition selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products, wherein said composition comprises a detectable amount of said nucleotide sequence.

21. A method of producing hybrid corn seeds comprising:

(a) planting seeds of a first inbred corn line comprising the DNA construct of claim 1 and seeds of a second inbred line having a genotype different from the first inbred corn line;

(b) cultivating corn plants resulting from said planting until time of flowering;

(c) emasculating said flowers of plants of one of the corn inbred lines;

(d) sexually crossing the two different inbred lines with each other; and

(e) harvesting the hybrid seed produced thereby.

22. The method according to claim 21 , wherein the plants of the first inbred corn line are the female parents.

23. The method according to claim 21 , wherein the plants of first inbred corn line are the male parents.

24. A method for producing a corn plant resistant to lepidopteran pests comprising:

(a) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant comprises event DP-004114-3 DNA, deposited under Accession No. PTA-11506 with American Type Culture Collection (ATCC), thereby producing a plurality of first generation progeny plants;

(b) selecting a first generation progeny plant that is resistant to lepidopteran insect infestation;

(c) selfing the first generation progeny plant, thereby producing a plurality of second generation progeny plants; and

(d) selecting from the second generation progeny plants, a plant that is resistant to lepidopteran pests;

wherein the second generation progeny plants comprise the DNA construct according to claim 1 .

25. The method of claim 21 further comprising backcrossing the second generation progeny plant of step (d) that comprises corn event DP-004114-3 DNA, deposited under Accession No. PTA-11506 with American Type Culture Collection (ATCC), to the parent plant that lacks the corn event DP-004114-3 DNA, thereby producing a backcross progeny plant that is resistant to at least western corn rootworm.

26. A method for producing a corn plant resistant to at least corn rootworm, said method comprising:

(a) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant is a corn event DP-004114-3, deposited under Accession No. PTA-11506 with American Type Culture Collection (ATCC), plant, thereby producing a plurality of first generation progeny plants;

(b) selecting a first generation progeny plant that is resistant to at least corn rootworm infestation;

(c) backcrossing the first generation progeny plant of step (b) with the parent plant that lacks corn event DP-004114-3 DNA, thereby producing a plurality of backcross progeny plants; and

(d) selecting from the backcross progeny plants, a plant that is resistant to at least corn rootworm infestation;

wherein the selected backcross progeny plant of step (d) comprises SEQ ID NO: 6.

27. The method according to claim 21 , wherein the plants of the first inbred corn line are the female parents or male parents.

28. Hybrid seed produced by the method of claim 21 .