IP Library › Granted Patent US 9,006,414
Granted Patent B2
US 9,006,414 · App. 12/973,821 · Granted Apr 14, 2015

Recombinant DNA for gene suppression

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Quick Facts
Patent No.
US 9,006,414
App. No.
12/973,821
Granted
Apr 14, 2015
Kind
B2
Abstract

Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.

Claims (10)

1. A recombinant DNA construct for suppression of at least one target gene which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element from at least one target gene and a sense-oriented DNA element comprising at least 21 nucleotides, wherein the sense-oriented DNA element is not more than about one-half the length of the anti-sense-oriented DNA element, and sense-oriented RNA transcribed from the sense-oriented DNA is complementary to and forms a double-stranded segment with the 5′-most end of anti-sense-oriented RNA transcribed from the anti-sense-oriented DNA element, wherein said transcribed RNA forms said double-stranded segment and an adjacent loop of anti-sense-oriented RNA for suppressing said at least one target gene.

2. The recombinant DNA construct of claim 1 wherein said anti-sense-oriented DNA element comprises, in series, segments from two or more genes targeted for suppression.

3. The recombinant DNA construct of claim 1 wherein said sense oriented DNA element is from one gene targeted for suppression and said anti-sense-oriented DNA is from two or more genes targeted for suppression.

4. The recombinant DNA construct of claim 1 wherein said sense-oriented DNA element and said anti-sense-oriented DNA are from a single gene targeted for suppression.

5. The recombinant DNA construct of claim 1 wherein said loop of anti-sense-oriented RNA is closed with a segment of double-stranded RNA.

6. The A recombinant DNA construct of claim 5 wherein one strand of said segment of double-stranded RNA is identical to mRNA from a gene targeted for suppression.

7. A recombinant DNA construct of claim 5 wherein said segment of double-stranded RNA is transcribed from DNA which is not from a gene targeted for suppression.

8. The recombinant DNA construct of claim 1 , wherein said sense-oriented DNA element comprises from 50 to 500 nucleotides.

9. The recombinant DNA construct of claim 1 , wherein said sense-oriented DNA element is not more than about one-third the length of the anti-sense-oriented DNA element.

10. The recombinant DNA construct of claim 1 , wherein said sense-oriented DNA element is not more than about one-quarter the length of the anti-sense-oriented DNA element.